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Literature summary extracted from

  • Madore, E.; Florentz, C.; Giege, R.; Sekine, S.; Yokoyama, S.; Lapointe, J.
    Effect of modified nucleotides on Escherichia coli tRNAGlu structure and on its aminoacylation by glutamyl-tRNA synthetase. Predominant and distinct roles of the mnm5 and s2 modifications of U34 (1999), Eur. J. Biochem., 266, 1128-1135.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.17 additional information construction and overexpression of tRNAGlu variants, the enzyme shows altered activity with the tRNA mutants, structural characterization Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.17 additional information
-
 additional information kinetics, diverse tRNAGlu mutants Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu Escherichia coli
-
 AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.17 Escherichia coli
-
 pure recombinant enzyme from overproducing strain DH5alpha
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.17 ATP + L-glutamate + tRNAGlu = AMP + diphosphate + L-glutamyl-tRNAGlu the major recognition element of the tRNAGlu is U at position 34 Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.17 ATP + L-glutamate + tRNAGlu
-
 Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?
6.1.1.17 ATP + L-glutamate + tRNAGlu the major recognition element of the tRNAGlu is U at position 34, activity with wild-type and mutant tRNAs, overview Escherichia coli AMP + diphosphate + L-glutamyl-tRNAGlu
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.17 GluRS
-
 Escherichia coli
6.1.1.17 Glutamyl-tRNA synthetase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.17 37
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.17 additional information
-
 additional information diverse tRNAGlu mutants Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.17 7.2
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.17 ATP
-
 Escherichia coli