BRENDA - Enzyme Database

Effect of modified nucleotides on Escherichia coli tRNAGlu structure and on its aminoacylation by glutamyl-tRNA synthetase. Predominant and distinct roles of the mnm5 and s2 modifications of U34

Madore, E.; Florentz, C.; Giege, R.; Sekine, S.; Yokoyama, S.; Lapointe, J.; Eur. J. Biochem. 266, 1128-1135 (1999)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
additional information
construction and overexpression of tRNAGlu variants, the enzyme shows altered activity with the tRNA mutants, structural characterization
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
kinetics, diverse tRNAGlu mutants
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Escherichia coli
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Escherichia coli
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.1.1.17
Escherichia coli
-
pure recombinant enzyme from overproducing strain DH5alpha
-
Reaction
EC Number
Reaction
Commentary
Organism
6.1.1.17
ATP + L-glutamate + tRNAGlu = AMP + diphosphate + L-glutamyl-tRNAGlu
the major recognition element of the tRNAGlu is U at position 34
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
651055
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651055
Escherichia coli
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
the major recognition element of the tRNAGlu is U at position 34, activity with wild-type and mutant tRNAs, overview
651055
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651055
Escherichia coli
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
6.1.1.17
37
-
assay at
Escherichia coli
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
diverse tRNAGlu mutants
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
7.2
-
assay at
Escherichia coli
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.17
ATP
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
6.1.1.17
ATP
-
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
6.1.1.17
additional information
construction and overexpression of tRNAGlu variants, the enzyme shows altered activity with the tRNA mutants, structural characterization
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
kinetics, diverse tRNAGlu mutants
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
Escherichia coli
-
AMP + diphosphate + L-glutamyl-tRNAGlu
-
Escherichia coli
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.1.1.17
ATP + L-glutamate + tRNAGlu
-
651055
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651055
Escherichia coli
?
6.1.1.17
ATP + L-glutamate + tRNAGlu
the major recognition element of the tRNAGlu is U at position 34, activity with wild-type and mutant tRNAs, overview
651055
Escherichia coli
AMP + diphosphate + L-glutamyl-tRNAGlu
-
651055
Escherichia coli
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
6.1.1.17
37
-
assay at
Escherichia coli
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.1.1.17
additional information
-
additional information
diverse tRNAGlu mutants
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1.1.17
7.2
-
assay at
Escherichia coli