BRENDA - Enzyme Database

T4 RNA ligase catalyzes the synthesis of dinucleoside polyphosphates

Atencia, E.A.; Madrid, O.; Gunther Sillero, M.A.; Sillero, A.; Eur. J. Biochem. 261, 802-811 (1999)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.5.1.3
tripolyphosphate
-
Escherichia virus T4
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.5.1.3
ATP + (ribonucleotide)n + (ribonucleotide)m
Escherichia virus T4
catalyzes the transfer of AMP from the E-AMP complex to diverse compounds, generating the product indicated between parentheses, ATP(Ap4A), NTP (Ap4N), P3 or P4 (p4A or p5A), ADP (Ap3A)
AMP + diphosphate + (ribonucleotide)n+m
-
Escherichia virus T4
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.5.1.3
Escherichia virus T4
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.5.1.3
0.06
-
-
Escherichia virus T4
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.5.1.3
ATP + (ribonucleotide)n + (ribonucleotide)m
-
651051
Escherichia virus T4
AMP + diphosphate + (ribonucleotide)n+m
-
651051
Escherichia virus T4
?
6.5.1.3
ATP + (ribonucleotide)n + (ribonucleotide)m
catalyzes the transfer of AMP from the E-AMP complex to diverse compounds, generating the product indicated between parentheses, ATP(Ap4A), NTP (Ap4N), P3 or P4 (p4A or p5A), ADP (Ap3A)
651051
Escherichia virus T4
AMP + diphosphate + (ribonucleotide)n+m
-
651051
Escherichia virus T4
?
6.5.1.3
ATP + ATP
-
651051
Escherichia virus T4
P1,P4-bis(5'-adenosyl) tetraphosphate + diphosphate
-
651051
Escherichia virus T4
?
6.5.1.3
ATP + ATP
-
651051
Escherichia virus T4
P1,P3-bis(5'-adenosyl) triphosphate + ?
-
651051
Escherichia virus T4
?
6.5.1.3
ATPgammaS + ATP
-
651051
Escherichia virus T4
P1,P4-bis(5'-adenosyl) tetraphosphate + thiodiphosphate
-
651051
Escherichia virus T4
?
6.5.1.3
GTP + ATP
-
651051
Escherichia virus T4
P1-(5'-adenosyl),P4-(5'-guanosyl) tetraphosphate + diphosphate
-
651051
Escherichia virus T4
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.5.1.3
0.017
-
GTP
pH 7.4, 37°C
Escherichia virus T4
6.5.1.3
0.055
-
ATP
pH 7.4, 30°C, ATP as acceptor of AMP, Ap4A synthesis
Escherichia virus T4
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5.1.3
7.4
-
-
Escherichia virus T4
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.5.1.3
6.5
8
around 70% of the maximum at pH 8.0
Escherichia virus T4
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.5.1.3
tripolyphosphate
-
Escherichia virus T4
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.5.1.3
ATP + (ribonucleotide)n + (ribonucleotide)m
Escherichia virus T4
catalyzes the transfer of AMP from the E-AMP complex to diverse compounds, generating the product indicated between parentheses, ATP(Ap4A), NTP (Ap4N), P3 or P4 (p4A or p5A), ADP (Ap3A)
AMP + diphosphate + (ribonucleotide)n+m
-
Escherichia virus T4
?
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
6.5.1.3
0.06
-
-
Escherichia virus T4
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.5.1.3
ATP + (ribonucleotide)n + (ribonucleotide)m
-
651051
Escherichia virus T4
AMP + diphosphate + (ribonucleotide)n+m
-
651051
Escherichia virus T4
?
6.5.1.3
ATP + (ribonucleotide)n + (ribonucleotide)m
catalyzes the transfer of AMP from the E-AMP complex to diverse compounds, generating the product indicated between parentheses, ATP(Ap4A), NTP (Ap4N), P3 or P4 (p4A or p5A), ADP (Ap3A)
651051
Escherichia virus T4
AMP + diphosphate + (ribonucleotide)n+m
-
651051
Escherichia virus T4
?
6.5.1.3
ATP + ATP
-
651051
Escherichia virus T4
P1,P4-bis(5'-adenosyl) tetraphosphate + diphosphate
-
651051
Escherichia virus T4
?
6.5.1.3
ATP + ATP
-
651051
Escherichia virus T4
P1,P3-bis(5'-adenosyl) triphosphate + ?
-
651051
Escherichia virus T4
?
6.5.1.3
ATPgammaS + ATP
-
651051
Escherichia virus T4
P1,P4-bis(5'-adenosyl) tetraphosphate + thiodiphosphate
-
651051
Escherichia virus T4
?
6.5.1.3
GTP + ATP
-
651051
Escherichia virus T4
P1-(5'-adenosyl),P4-(5'-guanosyl) tetraphosphate + diphosphate
-
651051
Escherichia virus T4
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
6.5.1.3
0.017
-
GTP
pH 7.4, 37°C
Escherichia virus T4
6.5.1.3
0.055
-
ATP
pH 7.4, 30°C, ATP as acceptor of AMP, Ap4A synthesis
Escherichia virus T4
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5.1.3
7.4
-
-
Escherichia virus T4
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
6.5.1.3
6.5
8
around 70% of the maximum at pH 8.0
Escherichia virus T4