EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.22.34 | DNA and amino acid sequence determination and analysis | Phaseolus vulgaris |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.34 | iodoacetamide | 11.6% remaining activity | Phaseolus vulgaris | |
3.4.22.34 | iodoacetate | 55.8 remaining activity | Phaseolus vulgaris | |
3.4.22.34 | additional information | no inhibition with EDTA, PMSF, pepstatin A, leupeptin, and E-64 | Phaseolus vulgaris | |
3.4.22.34 | N-ethylmaleimide | 16.6% remaining activity | Phaseolus vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | 38100 | - |
x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
3.4.22.34 | 53400 | - |
x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.34 | phaseolin + H2O | Phaseolus vulgaris | initiation of phaseolin proteolysis, enyme plays a key role in mobilization of phaseolin during and after kidney bean germination | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.34 | Phaseolus vulgaris | O24325 | legumain-like proteinase LLP | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.22.34 | no glycoprotein | but enzyme contains 1 putative N-glycosylation site at N326 | Phaseolus vulgaris |
3.4.22.34 | proteolytic modification | putative cleavage site is N367-E368 | Phaseolus vulgaris |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.34 | 44fold | Phaseolus vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.34 | cotyledon | germinated, developmental pattern | Phaseolus vulgaris | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | 7.04 | - |
purified enzyme, substrate casein | Phaseolus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.34 | casein + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
3.4.22.34 | phaseolin + H2O | - |
Phaseolus vulgaris | ? | - |
? | |
3.4.22.34 | phaseolin + H2O | initiation of phaseolin proteolysis, enyme plays a key role in mobilization of phaseolin during and after kidney bean germination | Phaseolus vulgaris | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.34 | ? | x * 38100, mature enzyme, SDS-PAGE, x * 53400, pre-proenzyme, SDS-PAGE | Phaseolus vulgaris |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.34 | asparaginyl-specific cysteine endopeptidase | - |
Phaseolus vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | 30 | - |
assay at | Phaseolus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.34 | 5.6 | - |
assay at | Phaseolus vulgaris |