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Literature summary extracted from

  • Mazauric, M.H.; Keith, G.; Logan, D.; Kreutzer, R.; Giege, R.; Kern, D.
    Glycyl-tRNA synthetase from Thermus thermophilus--wide structural divergence with other prokaryotic glycyl-tRNA synthetases and functional inter-relation with prokaryotic and eukaryotic glycylation systems (1998), Eur. J. Biochem., 251, 744-757.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.14 gene glyS, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli BL21(DE3) Thermus thermophilus

General Stability

EC Number General Stability Organism
6.1.1.14 glycyl-adenylate stabilizes the enzyme Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.14 additional information
-
 additional information kinetics for tRNAGly substrates of diverse prokaryotic and eukaryotic origins Thermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.14 Mg2+ required Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.14 51000
-
 x * 51000, SDS-PAGE Thermus thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly Thermus thermophilus
-
 AMP + diphosphate + glycyl-tRNAGly
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.14 Thermus thermophilus
-
 class II enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.14 recombinant enzyme from Escherichia coli, 10fold Thermus thermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.14 0.124
-
 purified recombinant enzyme Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.14 ATP + glycine + tRNAGly
-
 Thermus thermophilus AMP + diphosphate + glycyl-tRNAGly
-
 ?
6.1.1.14 ATP + glycine + tRNAGly substrate specificity with tRNAGly of diverse origins Thermus thermophilus AMP + diphosphate + glycyl-tRNAGly
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.14 ? x * 51000, SDS-PAGE Thermus thermophilus
6.1.1.14 More comparison of structures of enzymes of diverse origins Thermus thermophilus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.14 Glycine--tRNA ligase
-
 Thermus thermophilus
6.1.1.14 Glycyl translase
-
 Thermus thermophilus
6.1.1.14 Glycyl-transfer ribonucleate synthetase
-
 Thermus thermophilus
6.1.1.14 Glycyl-transfer ribonucleic acid synthetase
-
 Thermus thermophilus
6.1.1.14 Glycyl-transfer RNA synthetase
-
 Thermus thermophilus
6.1.1.14 Glycyl-tRNA synthetase
-
 Thermus thermophilus
6.1.1.14 GlyRS
-
 Thermus thermophilus
6.1.1.14 Synthetase, glycyl-transfer ribonucleate
-
 Thermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.14 37
-
 assay at Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.14 additional information
-
 additional information kcat for tRNAGly substrates of diverse prokaryotic and eukaryotic origins Thermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.14 7.2
-
 assay at Thermus thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.14 ATP
-
 Thermus thermophilus