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Literature summary extracted from

  • Matsuzawa, H.; Tokugawa, K.; Hamaoki, M.; Mizoguchi, M.; Taguchi, H.; Terada, I.; Kwon, S.T.; Ohta, T.
    Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1 (1988), Eur. J. Biochem., 171, 441-447.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.4.21.111 stable to denaturing reagents like 7M urea, 6M guanidine/HCl and 1% SDS Thermus aquaticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.111 microbial alkaline protease inhibitor 98% inhibition at 0.001 mg/ml Thermus aquaticus
3.4.21.111 Streptomyces subtilisin inhibitor 93% inhibition at 0.001 mg/ml Thermus aquaticus
3.4.21.111 Z-Ala-Gly-PheCH2Cl almost complete inhibition at 0.5 mM Thermus aquaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.111 20
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7, 50°C Thermus aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.111 28500
-
 SDS-PAGE Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.111 Thermus aquaticus
-
 YT-1
-
3.4.21.111 Thermus aquaticus YT-1
-
 YT-1
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.111 additional information
-
-
 Thermus aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.111 additional information preferentially hydrolyzes the ester bond of an alanine ester, but also shows relatively strong activity toward Gly, Trp, Phe and Tyr esters Thermus aquaticus ?
-
 ?
3.4.21.111 additional information preferentially hydrolyzes the ester bond of an alanine ester, but also shows relatively strong activity toward Gly, Trp, Phe and Tyr esters Thermus aquaticus YT-1 ?
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O synthetic substrate for elastase Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O synthetic substrate for elastase Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 oxidized insulin chain B + H2O cleavage sites are not specific Thermus aquaticus ?
-
 ?
3.4.21.111 oxidized insulin chain B + H2O cleavage sites are not specific Thermus aquaticus YT-1 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.111 70
-
 in the absence of Ca2+ Thermus aquaticus
3.4.21.111 80
-
 in the presence of Ca2+ Thermus aquaticus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.111 80
-
 Sr2+ and Ca2+ stabilize the enzyme to heat treatment Thermus aquaticus
3.4.21.111 90
-
 presence of Ca2+, 40% activity after 30 min Thermus aquaticus
3.4.21.111 95
-
 presence of Ca2+, 25% activity after 30 min Thermus aquaticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.111 2.6
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7, 50°C Thermus aquaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.111 10
-
-
 Thermus aquaticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.111 Thermus aquaticus pI-value above pH 9-10
-
 9