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Literature summary extracted from

  • Lescic, I.; Vukelic, B.; Majeric-Elenkov, M.; Saenger, W.; Abramic, M.
    Substrate specificity and effects of water-miscible solvents on the activity and stability of extracellular lipase from Streptomyces rimosus (2001), Enzyme Microb. Technol., 29, 548-553.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 1,4-dioxane 3.3fold activation at 2.5% v/v Streptomyces rimosus
3.1.1.3 acetone 1.9fold activation at 2.5% v/v Streptomyces rimosus
3.1.1.3 dimethyl sulfoxide 1.4fold activation at 2.5% v/v Streptomyces rimosus
3.1.1.3 ethanol 1.3fold activation at 2.5% v/v Streptomyces rimosus
3.1.1.3 N,N-Dimethylformamide 1.8fold activation at 2.5% v/v Streptomyces rimosus
3.1.1.3 tetrahydrofuran 2.9fold activation at 2.5% v/v Streptomyces rimosus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular
-
 Streptomyces rimosus
-
-

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 1,4-dioxane 18 h at 50% v/v, 100% stable Streptomyces rimosus
3.1.1.3 Acetone 18 h at 50% v/v, 80% remaining activity Streptomyces rimosus
3.1.1.3 acetonitrile 18 h at 50% v/v, 92% remaining activity Streptomyces rimosus
3.1.1.3 dimethyl sulfoxide 18 h at 50% v/v, 63% remaining activity Streptomyces rimosus
3.1.1.3 Ethanol 18 h at 50% v/v, 91% remaining activity Streptomyces rimosus
3.1.1.3 N,N-dimethylformamide 18 h at 50% v/v, 4% remaining activity Streptomyces rimosus
3.1.1.3 tetrahydrofuran 18 h at 50% v/v, no remaining activity, complete inactivation Streptomyces rimosus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Streptomyces rimosus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 170
-
 purified enzyme, substrate triolein Streptomyces rimosus
3.1.1.3 203
-
 purified enzyme, substrate poly(oxyethylene) sorbitan monooleate Streptomyces rimosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 2,3-dimercapto-1-propanol tributyrate + H2O regiospecificity study Streptomyces rimosus ?
-
 ?
3.1.1.3 additional information enzyme also performs transesterification reactions, regio- and enantioselctivity, overview, substrate specificity: enzyme hydrolyzes most efficiently medium chain length fatty acid glycerol esters, C8-C12, and shows a preference for esters of C16 and C18 unsaturated fatty acids over C16 and C18 saturated fatty acid esters, as well as for triacylglycerol substrates with cis double bond, e.g. triolein, versus trans double bonds, e.g. trielaidin, enzyme also utilizes different natural plant oils as substrates, overview Streptomyces rimosus ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Streptomyces rimosus dibutyrin + butyrate
-
 ?
3.1.1.3 tricaprin + H2O best substrate Streptomyces rimosus dicaprin + capriate
-
 ?
3.1.1.3 tricaprylin + H2O best substrate Streptomyces rimosus dicaprylin + caprylate
-
 ?
3.1.1.3 trielaidin + H2O trans C18:1 Streptomyces rimosus dielaidin + elaidiate
-
 ?
3.1.1.3 trilinolein + H2O
-
 Streptomyces rimosus dilinolein + linoleate
-
 ?
3.1.1.3 trilinolenin + H2O
-
 Streptomyces rimosus dilinolenin + linolenate
-
 ?
3.1.1.3 triolein + H2O cis C18:1 Streptomyces rimosus diolein + oleate main product is oleic acid, further products are 1,3-dioleylglycerol, 1,2-dioleylglycerol, 2,3-dioleylglycerol, and minor product is 1-monooleylglycerol ?
3.1.1.3 tripalmitin + H2O
-
 Streptomyces rimosus dipalmitin + palmitate
-
 ?
3.1.1.3 tripalmitolein + H2O
-
 Streptomyces rimosus dipalmitolein + palmitate
-
 ?
3.1.1.3 tristearin + H2O
-
 Streptomyces rimosus distearin + stearate
-
 ?
3.1.1.3 Tween 20 + H2O i.e. poly(oxyethylene) sorbitan monolaurate Streptomyces rimosus ?
-
 ?
3.1.1.3 Tween 40 + H2O i.e. poly(oxyethylene) sorbitan monopalmitate Streptomyces rimosus ?
-
 ?
3.1.1.3 Tween 60 + H2O i.e. poly(oxyethylene) sorbitan monostearate Streptomyces rimosus ?
-
 ?
3.1.1.3 Tween 80 + H2O i.e. poly(oxyethylene) sorbitan monooleate Streptomyces rimosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Streptomyces rimosus