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Substrate specificity and effects of water-miscible solvents on the activity and stability of extracellular lipase from Streptomyces rimosus

Lescic, I.; Vukelic, B.; Majeric-Elenkov, M.; Saenger, W.; Abramic, M.; Enzyme Microb. Technol. 29, 548-553 (2001)
No PubMed abstract available

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.3
1,4-dioxane
3.3fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
acetone
1.9fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
dimethyl sulfoxide
1.4fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
ethanol
1.3fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
N,N-Dimethylformamide
1.8fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
tetrahydrofuran
2.9fold activation at 2.5% v/v
Streptomyces rimosus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.3
extracellular
-
Streptomyces rimosus
-
-
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.1.3
1,4-dioxane
18 h at 50% v/v, 100% stable
Streptomyces rimosus
3.1.1.3
Acetone
18 h at 50% v/v, 80% remaining activity
Streptomyces rimosus
3.1.1.3
acetonitrile
18 h at 50% v/v, 92% remaining activity
Streptomyces rimosus
3.1.1.3
dimethyl sulfoxide
18 h at 50% v/v, 63% remaining activity
Streptomyces rimosus
3.1.1.3
Ethanol
18 h at 50% v/v, 91% remaining activity
Streptomyces rimosus
3.1.1.3
N,N-dimethylformamide
18 h at 50% v/v, 4% remaining activity
Streptomyces rimosus
3.1.1.3
tetrahydrofuran
18 h at 50% v/v, no remaining activity, complete inactivation
Streptomyces rimosus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.3
Streptomyces rimosus
-
-
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.3
170
-
purified enzyme, substrate triolein
Streptomyces rimosus
3.1.1.3
203
-
purified enzyme, substrate poly(oxyethylene) sorbitan monooleate
Streptomyces rimosus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.3
2,3-dimercapto-1-propanol tributyrate + H2O
regiospecificity study
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
additional information
enzyme also performs transesterification reactions, regio- and enantioselctivity, overview, substrate specificity: enzyme hydrolyzes most efficiently medium chain length fatty acid glycerol esters, C8-C12, and shows a preference for esters of C16 and C18 unsaturated fatty acids over C16 and C18 saturated fatty acid esters, as well as for triacylglycerol substrates with cis double bond, e.g. triolein, versus trans double bonds, e.g. trielaidin, enzyme also utilizes different natural plant oils as substrates, overview
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
tributyrin + H2O
-
651019
Streptomyces rimosus
dibutyrin + butyrate
-
651019
Streptomyces rimosus
?
3.1.1.3
tricaprin + H2O
best substrate
651019
Streptomyces rimosus
dicaprin + capriate
-
651019
Streptomyces rimosus
?
3.1.1.3
tricaprylin + H2O
best substrate
651019
Streptomyces rimosus
dicaprylin + caprylate
-
651019
Streptomyces rimosus
?
3.1.1.3
trielaidin + H2O
trans C18:1
651019
Streptomyces rimosus
dielaidin + elaidiate
-
651019
Streptomyces rimosus
?
3.1.1.3
trilinolein + H2O
-
651019
Streptomyces rimosus
dilinolein + linoleate
-
651019
Streptomyces rimosus
?
3.1.1.3
trilinolenin + H2O
-
651019
Streptomyces rimosus
dilinolenin + linolenate
-
651019
Streptomyces rimosus
?
3.1.1.3
triolein + H2O
cis C18:1
651019
Streptomyces rimosus
diolein + oleate
main product is oleic acid, further products are 1,3-dioleylglycerol, 1,2-dioleylglycerol, 2,3-dioleylglycerol, and minor product is 1-monooleylglycerol
651019
Streptomyces rimosus
?
3.1.1.3
tripalmitin + H2O
-
651019
Streptomyces rimosus
dipalmitin + palmitate
-
651019
Streptomyces rimosus
?
3.1.1.3
tripalmitolein + H2O
-
651019
Streptomyces rimosus
dipalmitolein + palmitate
-
651019
Streptomyces rimosus
?
3.1.1.3
tristearin + H2O
-
651019
Streptomyces rimosus
distearin + stearate
-
651019
Streptomyces rimosus
?
3.1.1.3
Tween 20 + H2O
i.e. poly(oxyethylene) sorbitan monolaurate
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
Tween 40 + H2O
i.e. poly(oxyethylene) sorbitan monopalmitate
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
Tween 60 + H2O
i.e. poly(oxyethylene) sorbitan monostearate
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
Tween 80 + H2O
i.e. poly(oxyethylene) sorbitan monooleate
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.3
1,4-dioxane
3.3fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
acetone
1.9fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
dimethyl sulfoxide
1.4fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
ethanol
1.3fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
N,N-Dimethylformamide
1.8fold activation at 2.5% v/v
Streptomyces rimosus
3.1.1.3
tetrahydrofuran
2.9fold activation at 2.5% v/v
Streptomyces rimosus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.1.3
extracellular
-
Streptomyces rimosus
-
-
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.1.3
1,4-dioxane
18 h at 50% v/v, 100% stable
Streptomyces rimosus
3.1.1.3
Acetone
18 h at 50% v/v, 80% remaining activity
Streptomyces rimosus
3.1.1.3
acetonitrile
18 h at 50% v/v, 92% remaining activity
Streptomyces rimosus
3.1.1.3
dimethyl sulfoxide
18 h at 50% v/v, 63% remaining activity
Streptomyces rimosus
3.1.1.3
Ethanol
18 h at 50% v/v, 91% remaining activity
Streptomyces rimosus
3.1.1.3
N,N-dimethylformamide
18 h at 50% v/v, 4% remaining activity
Streptomyces rimosus
3.1.1.3
tetrahydrofuran
18 h at 50% v/v, no remaining activity, complete inactivation
Streptomyces rimosus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.1.3
170
-
purified enzyme, substrate triolein
Streptomyces rimosus
3.1.1.3
203
-
purified enzyme, substrate poly(oxyethylene) sorbitan monooleate
Streptomyces rimosus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.3
2,3-dimercapto-1-propanol tributyrate + H2O
regiospecificity study
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
additional information
enzyme also performs transesterification reactions, regio- and enantioselctivity, overview, substrate specificity: enzyme hydrolyzes most efficiently medium chain length fatty acid glycerol esters, C8-C12, and shows a preference for esters of C16 and C18 unsaturated fatty acids over C16 and C18 saturated fatty acid esters, as well as for triacylglycerol substrates with cis double bond, e.g. triolein, versus trans double bonds, e.g. trielaidin, enzyme also utilizes different natural plant oils as substrates, overview
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
tributyrin + H2O
-
651019
Streptomyces rimosus
dibutyrin + butyrate
-
651019
Streptomyces rimosus
?
3.1.1.3
tricaprin + H2O
best substrate
651019
Streptomyces rimosus
dicaprin + capriate
-
651019
Streptomyces rimosus
?
3.1.1.3
tricaprylin + H2O
best substrate
651019
Streptomyces rimosus
dicaprylin + caprylate
-
651019
Streptomyces rimosus
?
3.1.1.3
trielaidin + H2O
trans C18:1
651019
Streptomyces rimosus
dielaidin + elaidiate
-
651019
Streptomyces rimosus
?
3.1.1.3
trilinolein + H2O
-
651019
Streptomyces rimosus
dilinolein + linoleate
-
651019
Streptomyces rimosus
?
3.1.1.3
trilinolenin + H2O
-
651019
Streptomyces rimosus
dilinolenin + linolenate
-
651019
Streptomyces rimosus
?
3.1.1.3
triolein + H2O
cis C18:1
651019
Streptomyces rimosus
diolein + oleate
main product is oleic acid, further products are 1,3-dioleylglycerol, 1,2-dioleylglycerol, 2,3-dioleylglycerol, and minor product is 1-monooleylglycerol
651019
Streptomyces rimosus
?
3.1.1.3
tripalmitin + H2O
-
651019
Streptomyces rimosus
dipalmitin + palmitate
-
651019
Streptomyces rimosus
?
3.1.1.3
tripalmitolein + H2O
-
651019
Streptomyces rimosus
dipalmitolein + palmitate
-
651019
Streptomyces rimosus
?
3.1.1.3
tristearin + H2O
-
651019
Streptomyces rimosus
distearin + stearate
-
651019
Streptomyces rimosus
?
3.1.1.3
Tween 20 + H2O
i.e. poly(oxyethylene) sorbitan monolaurate
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
Tween 40 + H2O
i.e. poly(oxyethylene) sorbitan monopalmitate
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
Tween 60 + H2O
i.e. poly(oxyethylene) sorbitan monostearate
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?
3.1.1.3
Tween 80 + H2O
i.e. poly(oxyethylene) sorbitan monooleate
651019
Streptomyces rimosus
?
-
651019
Streptomyces rimosus
?