Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bauer, M.; Griengl, H.; Steiner, W.
    Parameters influencing stability and activity of a S-hydroxynitrile lyase from Hevea brasiliensis in two-phase systems (1999), Enzyme Microb. Technol., 24, 514-522.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.1.2.47 half-life at pH 5 and 20°C is halved at a concentration of 2 mM benzaldehyde and 30 mM HCN, stability decreases drastically with increasing concentrations of benzaldehyde Hevea brasiliensis
4.1.2.47 half-life increases linearly with enzyme concentration in the aqueous buffer as well as in a two-phase system of buffer and dibutyl ether Hevea brasiliensis
4.1.2.47 influence of different organic solvents and solvent mixtures on the stability in two-phase systems of aqueous buffer and organic solvent Hevea brasiliensis
4.1.2.47 the enzyme shows especially high stability in mixtures of methyl-tert-butylether and hexane, 40:60 and 25:75 Hevea brasiliensis
4.1.2.47 the use of solvent mixtures with methyl-tert-butylether leads to better stability than the use of pure solvents Hevea brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.47 mandelonitrile
-
 Hevea brasiliensis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.2.47 dibutyl ether inactivates within a few min Hevea brasiliensis
4.1.2.47 Ethyl acetate inactivates within a few min Hevea brasiliensis
4.1.2.47 hexane inactivates within a few min, the use of solvent mixtures with methyl-tert-butylether leads to better stability than the use of pure solvents the enzyme shows especially high stability in mixtures of methyl-tert-butylether and hexane, 40:60 and 25:75 Hevea brasiliensis
4.1.2.47 methyl-tert-butylether the use of solvent mixtures with methyl-tert-butylether leads to better stability than the use of pure solvents the enzyme shows especially high stability in mixtures of methyl-tert-butylether and hexane, 40:60 and 25:75 Hevea brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.47 Hevea brasiliensis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.47 rac-mandelonitrile
-
 Hevea brasiliensis HCN + benzaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.47 (S)-Hydroxynitrile lyase
-
 Hevea brasiliensis
4.1.2.47 HbHNL
-
 Hevea brasiliensis
4.1.2.47 HNL
-
 Hevea brasiliensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.47 4.5
-
 below pH 4.5 enzyme is inactivated within a few min Hevea brasiliensis
4.1.2.47 5
-
 half-life in stirred solution is approximately 3.5 h Hevea brasiliensis