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Literature summary extracted from

  • Kanacher, T.; Schultz, A.; Linder, J.U.; Schultz, J.E.
    A GAF-domain-regulated adenylyl cyclase from Anabaena is a self-activating cAMP switch (2002), EMBO J., 21, 3672-3680.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.1 cAMP activation of recombinant cyaB1 via N-terminal GAF domains Anabaena sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 expression of cyaB1-1-859 AC holoenzyme and catalytic domain cyaB1-595-859 in Escherichia coli Anabaena sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.1 D650A mutation in cyaB1-595-859 catalytical domain, no activity Anabaena sp.
4.6.1.1 N728A mutation in cyaB1-595-859 catalytical domain, no activity Anabaena sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.1 0.004
-
 ATP 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 2 mM Mn2+ and 0.1 mM cAMP Anabaena sp.
4.6.1.1 0.011
-
 ATP 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 2 mM Mn2+ Anabaena sp.
4.6.1.1 0.024
-
 ATP 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 10 mM Mg2+ and 0.1 mM cAMP Anabaena sp.
4.6.1.1 0.038
-
 ATP 37°C, pH 7.5, recombinant cyaB1 AC, in the presence of 10 mM Mg2+ Anabaena sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.1 membrane
-
 Anabaena sp. 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.1 Mg2+ supports activity of recombinant cyaB1 AC better than Mn2+ Anabaena sp.
4.6.1.1 Mn2+ activation of recombinant cyaB1 AC Anabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.1 ATP Anabaena sp.
-
 3',5'-cyclic AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Anabaena sp. P94181 PCC 7120, class III type adeylyl caclase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1 recombinant cyaB1 Anabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Anabaena sp. 3',5'-cyclic AMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 AC
-
 Anabaena sp.
4.6.1.1 adenylyl cyclase
-
 Anabaena sp.
4.6.1.1 cyaB1
-
 Anabaena sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.1 40
-
 recombinant cyaB1 AC holoenzyme Anabaena sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.1 7.5
-
 recombinant cyaB1 AC holoenzyme Anabaena sp.