EC Number | Crystallization (Comment) | Organism |
---|---|---|
6.1.1.16 | purified recombinant free enzyme or cysteine and ATP complexed enzyme, hanging-drop vapour diffusion method, 17°C, 0.001 ml protein solution: 6 mg/ml enzyme, 10 mM HEPES, pH 7.4, 50 mM NaCl, 1 mM DTT, 5 mM MgCl2, 5 mM ATP, 10 mM cysteine, plus equal volume of reservoir solution: 0.1 M sodium cacodylate, pH 6.5, 15-17% PEG 8000, 0.2 M magnesium acetate, 2% tert-butanol, 1-3 weeks, cysteine but not ATP is required for crystal growth, X-ray diffraction structure determination at 2.3-3.0 A resolution, and analysis | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.16 | Zn2+ | 1 ion bound at the base of the active site cleft, important for tRNA substrate specificity, single direct interaction with cysteine thiolate substrate | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.16 | ATP + L-cysteine + tRNACys | Escherichia coli | - |
AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
6.1.1.16 | Escherichia coli | P21888 | class I enzyme | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
6.1.1.16 | ATP + L-cysteine + tRNACys = AMP + diphosphate + L-cysteinyl-tRNACys | substrate binding mechanism, induced fit | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
6.1.1.16 | ATP + L-cysteine + tRNACys | - |
Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? | |
6.1.1.16 | ATP + L-cysteine + tRNACys | enzyme is highly specific for L-cysteine and does not possess the editing activity characteristic for other tRNA synthetases | Escherichia coli | AMP + diphosphate + L-cysteinyl-tRNACys | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
6.1.1.16 | monomer | - |
Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
6.1.1.16 | CysRS | - |
Escherichia coli |
6.1.1.16 | Cysteine translase | - |
Escherichia coli |
6.1.1.16 | Cysteine--tRNA ligase | - |
Escherichia coli |
6.1.1.16 | Cysteinyl-transfer ribonucleate synthetase | - |
Escherichia coli |
6.1.1.16 | Cysteinyl-transfer RNA synthetase | - |
Escherichia coli |
6.1.1.16 | Cysteinyl-tRNA synthetase | - |
Escherichia coli |
6.1.1.16 | Synthetase, cysteinyl-transfer ribonucleate | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
6.1.1.16 | ATP | - |
Escherichia coli |