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Literature summary extracted from

  • Kaminska, M.; Deniziak, M.; Kerjan, P.; Barciszewski, J.; Mirande, M.
    A recurrent general RNA binding domain appended to plant methionyl-tRNA synthetase acts as a cis-acting cofactor for aminoacylation (2000), EMBO J., 19, 6908-6917.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.10 DNA and amino acid sequence determination, overexpression of full-length wild-type and a deleted version lacking the C-terminal EMAPII-like domain in Escherichia coli BL21(DE3) Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.10 0.00066
-
 tRNAMet in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, wild-type enzyme Oryza sativa
6.1.1.10 0.0007
-
 tRNA fraction from Saccharomyces cerevisiae pH 7.5, 25°C, wild-type enzyme Oryza sativa
6.1.1.10 0.00075
-
 tRNA fraction from Saccharomyces cerevisiae pH 7.5, 25°C, truncated mutant Oryza sativa
6.1.1.10 0.0065
-
 tRNAMet in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, truncated mutant Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.10 cytoplasm
-
 Oryza sativa 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.10 62000
-
 truncated enzyme, gel filtration Oryza sativa
6.1.1.10 66500
-
 1 * 66500, truncated enzyme, SDS-PAGE, 1 * 89700, wild-type enzyme, SDS-PAGE Oryza sativa
6.1.1.10 89700
-
 1 * 66500, truncated enzyme, SDS-PAGE, 1 * 89700, wild-type enzyme, SDS-PAGE Oryza sativa
6.1.1.10 110000
-
 wild-type enzyme, gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNAMet Oryza sativa
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.10 Oryza sativa Q9ZTS1 enzyme contains a supplementary C-terminal domain related to endothelial monocyte activating polypeptide II, i.e. EMAPII, a cytokine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.10 recombinant enzyme from Escherichia coli Oryza sativa

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.10 ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet the amino acid acceptor arm of tRNAMet binds to the plant enzyme Oryza sativa
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.10 ATP + L-methionine + tRNA fraction from Saccharomyces cerevisiae
-
 Oryza sativa ?
-
 ?
6.1.1.10 ATP + L-methionine + tRNAAsp only the wild-type enzyme, not the C-terminally deleted enzyme mutant Oryza sativa AMP + diphosphate + L-methionyl-tRNAAsp
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet
-
 Oryza sativa AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
6.1.1.10 ATP + L-methionine + tRNAMet the EMAPII-like C-terminal appendix domain provides the enzyme with non-specific tRNA binding properties, the deleted enzyme form missing this domain is therefore more specific and shows a 10fold lower Km for the tRNA substrate, enzyme is also active with tRNAMet from Saccharomyces cerevisiae Oryza sativa AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.10 monomer 1 * 66500, truncated enzyme, SDS-PAGE, 1 * 89700, wild-type enzyme, SDS-PAGE Oryza sativa
6.1.1.10 More at high protein concentration the enzyme can form a dimer in vitro Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
6.1.1.10 Methionine translase
-
 Oryza sativa
6.1.1.10 Methionine--tRNA ligase
-
 Oryza sativa
6.1.1.10 Methionyl tRNA synthetase
-
 Oryza sativa
6.1.1.10 Methionyl-transfer ribonucleate synthetase
-
 Oryza sativa
6.1.1.10 Methionyl-transfer ribonucleic acid synthetase
-
 Oryza sativa
6.1.1.10 Methionyl-transfer RNA synthetase
-
 Oryza sativa
6.1.1.10 MetRS
-
 Oryza sativa
6.1.1.10 Synthetase, methionyl-transfer ribonucleate
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.10 25
-
 assay at Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.10 0.26
-
 tRNA fraction from Saccharomyces cerevisiae pH 7.5, 25°C, truncated mutant Oryza sativa
6.1.1.10 0.29
-
 tRNAMet in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, wild-type enzyme Oryza sativa
6.1.1.10 0.36
-
 tRNAMet in vitro synthesized tRNAMet from Saccharomyces cerevisiae, pH 7.5, 25°C, truncated mutant Oryza sativa
6.1.1.10 0.53
-
 tRNA fraction from Saccharomyces cerevisiae pH 7.5, 25°C, wild-type enzyme Oryza sativa
6.1.1.10 6.08
-
 tRNA fraction from Saccharomyces cerevisiae pH 7.5, 25°C, wild-type enzyme Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.10 7.5
-
 assay at Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.10 ATP
-
 Oryza sativa