Literature summary extracted from
Jedrzejas, M.J.; Chander, M.; Setlow, P.; Krishnasamy, G.
Structure and mechanism of action of a novel phosphoglycerate mutase from Bacillus stearothermophilus (2000), EMBO J., 19, 1419-1431.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
5.4.2.12 |
expression in Escherichia coli |
Geobacillus stearothermophilus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.4.2.12 |
crystals of iPGM complexed with 3-phosphoglycerate and Mn2+ at 1.9 A resolution |
Geobacillus stearothermophilus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
5.4.2.12 |
D12N |
no activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H123N |
3% of wild-type activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H125N |
67% of wild-type activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H128N |
20% of wild-type activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H407N |
no activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H42N |
91% of wild-type activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H42N/H128N |
8% of wild-type activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H445N |
18% of wild-type activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H462N |
0.3% of wild-type activity |
Geobacillus stearothermophilus |
5.4.2.12 |
H66N |
2% of wild-type activity |
Geobacillus stearothermophilus |
5.4.2.12 |
R261L |
no activity |
Geobacillus stearothermophilus |
5.4.2.12 |
S62A |
no activity |
Geobacillus stearothermophilus |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
5.4.2.12 |
Mn2+ |
absolutely required for activity, maximal activation at 1 mM |
Geobacillus stearothermophilus |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.4.2.12 |
2-phospho-D-glycerate |
Geobacillus stearothermophilus |
- |
3-phospho-D-glycerate |
- |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.2.12 |
Geobacillus stearothermophilus |
Q9X519 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.2.12 |
recombinant iPGM |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.4.2.12 |
2-phospho-D-glycerate |
- |
Geobacillus stearothermophilus |
3-phospho-D-glycerate |
- |
r |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.4.2.12 |
iPGM |
- |
Geobacillus stearothermophilus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
5.4.2.12 |
8 |
- |
- |
Geobacillus stearothermophilus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
5.4.2.12 |
6 |
8 |
2% and 22% of maximal activity at pH 6.0 and pH 7.0, respectively, at 1 mM Mn2+ |
Geobacillus stearothermophilus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
5.4.2.12 |
additional information |
2,3-bisphosphoglycerare independend PGM |
Geobacillus stearothermophilus |
|