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Literature summary extracted from

  • Lee, J.Y.; Chang, C.; Song, H.K.; Moon, J.; Yang, J.K.; Kim, H.K.; Kwon, S.T.; Suh, S.W.
    Crystal structure of NAD+-dependent DNA ligase: modular architecture and functional implications (2000), EMBO J., 19, 1119-1129.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
6.5.1.2 medicine potential target for antibiotics Thermus filiformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.2 native and selenomethionine-substituted enzyme Thermus filiformis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.5.1.2 vapor diffusion method Thermus filiformis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.2 Mg2+ required Thermus filiformis
6.5.1.2 Zn2+ 1 mol per mol of enzyme, Cys406, Cys409, Cys422 and Cys427 form a zinc binding motif Thermus filiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.2 75940
-
 calculated from DNA-sequence Thermus filiformis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m Thermus filiformis DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.2 Thermus filiformis Q9ZHI0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.2
-
 Thermus filiformis

Reaction

EC Number Reaction Comment Organism Reaction ID
6.5.1.2 ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta-nicotinamide D-nucleotide 3 steps of reaction: 1. adenylation of the ligase in the presence of NAD+, 2. transferring the adenylate moiety to the 5'-phosphate of the nicked DNA substrate, 3. sealing the nick through the formation of a phosphodiester bond Thermus filiformis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Thermus filiformis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?
6.5.1.2 NAD+ + (deoxyribonucleotide)n + (deoxyribonucleotide)m DNA-repair, phosphodiester bond formation between adjacent 5'-phosphate and 3'-hydroxyl groups in double-stranded DNA Thermus filiformis AMP + nicotinamide nucleotide + (deoxyribonucleotide)n+m
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.2 monomer
-
 Thermus filiformis

Cofactor

EC Number Cofactor Comment Organism Structure
6.5.1.2 NAD+ required Thermus filiformis