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Literature summary extracted from

  • Jungmann, J.; Munro, S.
    Multi-protein complexes in the cis Golgi of Saccharomyces cerevisiae with alpha-1,6-mannosyltransferase activity (1998), EMBO J., 17, 423-434.
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.232 Golgi apparatus Mnn9p, primarily localized to the cis Golgi, type II transmembrane protein Saccharomyces cerevisiae 5794
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.232 additional information Saccharomyces cerevisiae two distinct Mnn9p-containing multi-protein complexes are responsible for the synthesis and initial branching of the long alpha-1,6-linked backbone of the hypermannose structure attached to many yeast glycoproteins, the long alpha-1,6-linked backbone is attached to an initial alpha-1,6-mannose residue transferred to the core structure by Och1p ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.232 Saccharomyces cerevisiae
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-
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.232 GDP-mannose + alpha-1,6-D-mannobiose two distinct Mnn9p-containing multi-protein complexes add multiple mannosyl residues forming a series of poly-mannose structures in vitro, no substrate of Och1p Saccharomyces cerevisiae ? formation of a series of poly-mannose structures, which contain mainly alpha-1,6- and some additional alpha-1,2-linked mannoses ?
2.4.1.232 GDP-mannose + oligosaccharide acceptor Mnn9p is associated in two distinct multi-protein complexes, both having alpha-1,6-mannosyltransferase activity, they add multiple mannosyl residues forming hypermannose structures, Och1p adds the initiating alpha-1,6-mannose residue to an N-linked Man8GlcNAc2 structure, the Mnn9p-containing protein complexes add further alpha-1,6-mannose residues synthesizing the backbone of the outer chain of N-linked glycans Saccharomyces cerevisiae GDP + alpha-mannosyl-1,6-oligosaccharide
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 ?
2.4.1.232 additional information Mnn9p, no acceptor: alpha-1,2-mannobiose Saccharomyces cerevisiae ?
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 ?
2.4.1.232 additional information two distinct Mnn9p-containing multi-protein complexes are responsible for the synthesis and initial branching of the long alpha-1,6-linked backbone of the hypermannose structure attached to many yeast glycoproteins, the long alpha-1,6-linked backbone is attached to an initial alpha-1,6-mannose residue transferred to the core structure by Och1p Saccharomyces cerevisiae ?
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.232 Mnn9p Saccharomyces cerevisiae, part of two elongating alpha-1,6-mannosyltransferase complexes Saccharomyces cerevisiae
2.4.1.232 Och1p Saccharomyces cerevisiae, initiating alpha-1,6-mannosyltransferase Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.232 30
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 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.232 7.2
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 assay at Saccharomyces cerevisiae