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Literature summary extracted from

  • Leonhard, K.; Herrmann, J.M.; Stuart, R.A.; Mannhaupt, G.; Neupert, W.; Langer, T.
    AAA proteases with catalytic sites on opposite membrane surfaces comprise a proteolytic system for the ATP-dependent degradation of inner membrane proteins in mitochondria (1996), EMBO J., 15, 4218-4229.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B19 expression of wild-type and mutant E41Q in deficient mutant cell DELTAyme1, complementation only by the wild-type Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B18 E614Q site-directed mutagenesis, exchange of active site residue, stongly reduced activity Saccharomyces cerevisiae
3.4.24.B19 E541Q site-directed mutagenesis, exchange in the active site, no complementation of deficient mutant cell DELTAyme1 Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B19 EDTA strong Saccharomyces cerevisiae
3.4.24.B19 o-phenanthroline strong Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B18 mitochondrial inner membrane inner side, exposes catalytic sites to the mitochondrial matrix Saccharomyces cerevisiae 5743
-
3.4.24.B18 mitochondrion
-
 Saccharomyces cerevisiae 5739
-
3.4.24.B19 mitochondrial inner membrane integral, a large domain is exposed to the intermembrane space Saccharomyces cerevisiae 5743
-
3.4.24.B19 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B18 protein + H2O Saccharomyces cerevisiae
-
 peptides
-
 ?
3.4.24.B19 protein + H2O Saccharomyces cerevisiae proteolytic activity of the i-AAA protease is required for the maintenance of mitochondrial function at high temperature, pointing to an important role in mitochondrial biogenesis peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B18 Saccharomyces cerevisiae
-
 in complex with Yme1p, EC 3.4.24.B19, forming i-AAA protease
-
3.4.24.B19 Saccharomyces cerevisiae
-
 in complex with m-AAA protease Yta12p, EC 3.4.24.B18, complex is termed i-AAA protease
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.B18 proteolytic degradation of proteins conserved metal-binding site HEXXH, selective degradation, mechanism Saccharomyces cerevisiae
a
3.4.24.B19 proteolytic degradation of proteins conserved metal-binding site HEXXH, selective degradation, mechanism Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B18 hybrid protein + H2O hybrid protein of subunit 2 of cytochrome oxidase residues 1-74, mouse dihydrofolate reductase, and mitochondrial presequence, residues 1-66, of subunit 9 of the ATPase of Neurospora crassa, in vitro import into the mitochondrion Saccharomyces cerevisiae 2 peptide fragments f2 and f3 product characterization ?
3.4.24.B18 additional information Yta12p enzyme requires YTA10-12 complex formation for activity Saccharomyces cerevisiae ?
-
 ?
3.4.24.B18 protein + H2O
-
 Saccharomyces cerevisiae peptides
-
 ?
3.4.24.B18 residues 1-74 of subunit 2 of cytochrome oxidase + H2O two-step procedure, in vitro import into the mitochondrion Saccharomyces cerevisiae ?
-
 ?
3.4.24.B19 hybrid protein of subunit 2 of cytochrome oxidase residues 1-74, mouse dihydrofolate reductase, and mitochondrial presequence, residues 1-66, of subunit 9 of the ATPase of Neurospora crassa + H2O in vitro import into the mitochondrion Saccharomyces cerevisiae 4 peptide fragments f1-f4 product characterization ?
3.4.24.B19 protein + H2O proteolytic activity of the i-AAA protease is required for the maintenance of mitochondrial function at high temperature, pointing to an important role in mitochondrial biogenesis Saccharomyces cerevisiae peptides
-
 ?
3.4.24.B19 residues 1-74 of subunit 2 of cytochrome oxidase + H2O two-step procedure, in vitro import into the mitochondrion Saccharomyces cerevisiae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B18 M41.003 Merops-ID Saccharomyces cerevisiae
3.4.24.B18 YTA12p
-
 Saccharomyces cerevisiae
3.4.24.B19 AAA protease
-
 Saccharomyces cerevisiae
3.4.24.B19 M41.004 Merops-ID Saccharomyces cerevisiae
3.4.24.B19 YME1
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B18 25
-
 assay at Saccharomyces cerevisiae
3.4.24.B19 25
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
3.4.24.B18 ATP dependent on Saccharomyces cerevisiae
3.4.24.B19 ATP dependent on Saccharomyces cerevisiae