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Literature summary extracted from

  • Sugiyama, A.; Shiraki, M.; Kobayashi, T.; Morikawa, G.; Yamamoto, M.; Yamaoka, M.; Saito, T.
    Cloning and sequencing of an intracellular D(-)-3-hydroxybutyrate oligomer hydrolase from Acidovorax sp. strain SA1 and purification of the enzyme (2002), Curr. Microbiol., 45, 123-127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.22 gene i3HBOH, DNA sequence determination and analysis, expression in Escherichia coli Acidovorax sp. SA1

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.22 S102A site-directed mutagenesis, inactive mutant Acidovorax sp. SA1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.22 1.3
-
 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate pH 8.0, 30°C Acidovorax sp. SA1
3.1.1.22 1.5
-
 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate pH 8.0, 30°C Acidovorax sp. SA1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.22 intracellular
-
 Acidovorax sp. SA1 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.22 Acidovorax sp. SA1 Q9F1T8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.22 native from strain SA1 and recombinant from Escherichia coli Acidovorax sp. SA1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
-
 Acidovorax sp. SA1 (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O
-
 Acidovorax sp. SA1 (R)-3-hydroxybutanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.22 D(-)-3-hydroxybutyrate oligomer hydrolase
-
 Acidovorax sp. SA1