BRENDA - Enzyme Database

Phospholipase D regulation and localization is dependent upon a phosphatidylinositol 4,5-bisphosphate-specific PH domain

Hodgkin, M.N.; Masson, M.R.; Powner, D.; Saqib, K.M.; Ponting, C.P.; Wakelam, M.J.O.; Curr. Biol. 10, 43-46 (2000)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
phosphatidylinositol
-
Homo sapiens
3.1.4.4
phosphatidylinositol-3,4,5-trisphosphate
effective activator
Homo sapiens
3.1.4.4
phosphatidylinositol-3,4-diphosphate
very effective activator
Homo sapiens
3.1.4.4
phosphatidylinositol-3,5-diphosphate
effective activator
Homo sapiens
3.1.4.4
phosphatidylinositol-3-phosphate
effective activator
Homo sapiens
3.1.4.4
phosphatidylinositol-4,5-diphosphate
very effective activator
Homo sapiens
3.1.4.4
phosphatidylserine
-
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.4
F250S
dramatic reduction of enzyme activity
Homo sapiens
3.1.4.4
K228R
dramatic reduction of enzyme activity
Homo sapiens
3.1.4.4
additional information
deletion of pleckstrin homology domain, disruption of normal cellular localization, loss of enzyme activity
Homo sapiens
3.1.4.4
S230F
dramatic reduction of enzyme activity
Homo sapiens
3.1.4.4
W253C
almost complete loss of enzyme activity
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Homo sapiens
-
isozymes PLD1, PLD2
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
-
650910
Homo sapiens
choline + phosphatidate
-
650910
Homo sapiens
?
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.4
phosphatidylinositol
-
Homo sapiens
3.1.4.4
phosphatidylinositol-3,4,5-trisphosphate
effective activator
Homo sapiens
3.1.4.4
phosphatidylinositol-3,4-diphosphate
very effective activator
Homo sapiens
3.1.4.4
phosphatidylinositol-3,5-diphosphate
effective activator
Homo sapiens
3.1.4.4
phosphatidylinositol-3-phosphate
effective activator
Homo sapiens
3.1.4.4
phosphatidylinositol-4,5-diphosphate
very effective activator
Homo sapiens
3.1.4.4
phosphatidylserine
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.4.4
F250S
dramatic reduction of enzyme activity
Homo sapiens
3.1.4.4
K228R
dramatic reduction of enzyme activity
Homo sapiens
3.1.4.4
additional information
deletion of pleckstrin homology domain, disruption of normal cellular localization, loss of enzyme activity
Homo sapiens
3.1.4.4
S230F
dramatic reduction of enzyme activity
Homo sapiens
3.1.4.4
W253C
almost complete loss of enzyme activity
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
phosphatidylcholine + H2O
-
650910
Homo sapiens
choline + phosphatidate
-
650910
Homo sapiens
?