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Literature summary extracted from

  • Miled, N.; Bussetta, C.; De Caro, A.; Riviere, M.; Berti, L.; Canaan, S.
    Importance of the lid and cap domains for the catalytic activity of gastric lipases (2003), Comp. Biochem. Physiol. B, 136B, 131-138.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 expression of wild-type enzyme and mutants lacking the cap and the lid domain via baculovirus transfection in insect cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 additional information construction of mutants lacking the cap and the lid domain by oligonucleotide-directed mutagenesis, influencing the expresion level, stability, activity, and reaction kinetics Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular secretion in the stomach Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 32000
-
 x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE Homo sapiens
3.1.1.3 43000
-
 x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE Homo sapiens
3.1.1.3 47000
-
 x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Homo sapiens
-
 gastric lipase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant wild-type and mutant enzymes from insect cells, mutant lacking the cap domain is only stable at acidic pH value during purification Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.3 triacylglycerol + H2O = diacylglycerol + a carboxylate catalytic serine residue, deeply buried under a domain called the extrusion domain, which is composed of a cap and a lid segmentof 58 amino acids, catalytic reaction mechanism Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.3 stomach
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.08
-
 purified recombinant mutant enzyme lacking the cap domain, substrate triolein Homo sapiens
3.1.1.3 0.5
-
 purified recombinant mutant enzyme lacking the lid domain, substrate triolein Homo sapiens
3.1.1.3 30
-
 purified recombinant wild-type enzyme, substrate triolein Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 triolein + H2O
-
 Homo sapiens diolein + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 47000, wild-type enzyme, SDS-PAGE, x * 43000, lid domain lacking mutant, SDS-PAGE, x * 32000, cap domain lacking mutant, SDS-PAGE Homo sapiens
3.1.1.3 More epitope mapping Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 HGL
-
 Homo sapiens
3.1.1.3 More the enzyme belongs to the alpha/beta hydrolase fold family Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 5
-
 assay at Homo sapiens