BRENDA - Enzyme Database

Enolase from Candida albicans--purification and characterization

Kustrzeba-Wojcicka, I.; Golczak, M.; Comp. Biochem. Physiol. B 126, 109-120 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
noncompetitive in the presence of phosphate, competitive in the absence of phosphate
Candida albicans
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.38
-
2-phospho-D-glycerate
pH 6.8, 30ºC
Candida albicans
4.2.1.11
0.95
-
phosphoenolpyruvate
pH 6.8, 30ºC
Candida albicans
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
required for activity, can be replaced by Mn2+ or Zn2+, but lower activity is observed
Candida albicans
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
x * 46000, SDS-PAGE
Candida albicans
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Candida albicans
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
to homogeneity, using ammonium sulfate precipitation and DEAE-Sephadex column chromatography
Candida albicans
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
35
-
pH 6.8, 30ºC
Candida albicans
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
650872
Candida albicans
phosphoenolpyruvate
-
650872
Candida albicans
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46000, SDS-PAGE
Candida albicans
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.11
50
-
heat shock protein, 50% of the activity retained after 1 h of incubation, 30% of the activity retained after 2 h of incubation
Candida albicans
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
-
Candida albicans
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6.5
7
steep decrease in activity below pH 6.5, 50% of the maximal activity retained above pH 7.0
Candida albicans
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
10
-
F-
pH 6.8, 30ºC, in the absence of phosphate
Candida albicans
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
F-
noncompetitive in the presence of phosphate, competitive in the absence of phosphate
Candida albicans
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.2.1.11
10
-
F-
pH 6.8, 30ºC, in the absence of phosphate
Candida albicans
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.38
-
2-phospho-D-glycerate
pH 6.8, 30ºC
Candida albicans
4.2.1.11
0.95
-
phosphoenolpyruvate
pH 6.8, 30ºC
Candida albicans
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
required for activity, can be replaced by Mn2+ or Zn2+, but lower activity is observed
Candida albicans
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
x * 46000, SDS-PAGE
Candida albicans
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
to homogeneity, using ammonium sulfate precipitation and DEAE-Sephadex column chromatography
Candida albicans
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
35
-
pH 6.8, 30ºC
Candida albicans
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
650872
Candida albicans
phosphoenolpyruvate
-
650872
Candida albicans
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46000, SDS-PAGE
Candida albicans
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.2.1.11
50
-
heat shock protein, 50% of the activity retained after 1 h of incubation, 30% of the activity retained after 2 h of incubation
Candida albicans
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
-
Candida albicans
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6.5
7
steep decrease in activity below pH 6.5, 50% of the maximal activity retained above pH 7.0
Candida albicans