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Literature summary extracted from

  • Fernandez-Centeno, E.; Heredia, C.F.
    2',3'-Cyclic nucleotide 2'-phosphodiesterase from Fusarium culmorum (2000), Comp. Biochem. Physiol. B, 125, 161-167.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.1.4.16 activity is not affected by several cycles of freezing and thawing Fusarium culmorum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.16 Co2+ 0.1 mM, 80% inhibition Fusarium culmorum
3.1.4.16 Cu2+ strong inhibition at micromolar concentrations, no effect on enzyme stability Fusarium culmorum
3.1.4.16 Mn2+ 0.5 mM, 50% inhibition Fusarium culmorum
3.1.4.16 additional information not inhibited by EDTA Fusarium culmorum
3.1.4.16 Zn2+ strong inhibition at micromolar concentrations, no effect on enzyme stability Fusarium culmorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.16 0.1
-
 cyclic 2',3'-GMP pH 7.5, 30°C Fusarium culmorum
3.1.4.16 0.3
-
 cyclic 2',3'-AMP pH 7.5, 30°C Fusarium culmorum
3.1.4.16 0.6
-
 cyclic 2',3'-CMP pH 7.5, 30°C Fusarium culmorum
3.1.4.16 2
-
 cyclic 2',3'-UMP pH 7.5, 30°C Fusarium culmorum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.16 additional information no requirement for metal ions Fusarium culmorum

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.16 Fusarium culmorum
-
 CECT 2148
-
3.1.4.16 Fusarium culmorum CECT 2148
-
 CECT 2148
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.16 mycelium
-
 Fusarium culmorum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.16 0.025
-
 pH 7.5, 30°C, crude extract Fusarium culmorum

Storage Stability

EC Number Storage Stability Organism
3.1.4.16 -20°C, several weeks, stable Fusarium culmorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.16 cyclic 2',3'-AMP + H2O
-
 Fusarium culmorum 3'-AMP the only major reaction product ?
3.1.4.16 cyclic 2',3'-AMP + H2O
-
 Fusarium culmorum CECT 2148 3'-AMP the only major reaction product ?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
 Fusarium culmorum 3'-CMP the only major reaction product ?
3.1.4.16 cyclic 2',3'-CMP + H2O
-
 Fusarium culmorum CECT 2148 3'-CMP the only major reaction product ?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
 Fusarium culmorum 3'-GMP the only major reaction product ?
3.1.4.16 cyclic 2',3'-GMP + H2O
-
 Fusarium culmorum CECT 2148 3'-GMP the only major reaction product ?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
 Fusarium culmorum 3'-UMP the only major reaction product ?
3.1.4.16 cyclic 2',3'-UMP + H2O
-
 Fusarium culmorum CECT 2148 3'-UMP the only major reaction product ?
3.1.4.16 additional information not: 3’,5’-nucleoside monophosphates, nucleoside 5’-, 3’- or 2’-monophosphates, tRNA, polyU Fusarium culmorum ?
-
 ?
3.1.4.16 additional information not: 3’,5’-nucleoside monophosphates, nucleoside 5’-, 3’- or 2’-monophosphates, tRNA, polyU Fusarium culmorum CECT 2148 ?
-
 ?
3.1.4.16 nucleoside 2',3'-cyclic phosphate + H2O
-
 Fusarium culmorum nucleoside 3'-phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.16 30
-
 assay at Fusarium culmorum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.16 40 80 very stable to heat, medium concentrate of enzyme, 5 min, stable Fusarium culmorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.16 7 8
-
 Fusarium culmorum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.16 8
-
 at pH-values greater than 8 activity declines sharply Fusarium culmorum