BRENDA - Enzyme Database

Hydrolytic and transphosphatidylation activities of phospholipase D from Savoy cabbage towards lysophosphatidylcholine

Virto, C.; Svensson, I.; Adlercreutz, P.; Chem. Phys. Lipids 106, 41-51 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
CTAB
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
Brassica oleracea var. capitata
3.1.4.4
SDS
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
Brassica oleracea var. capitata
3.1.4.4
Triton X-100
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
Brassica oleracea var. capitata
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
1
-
lysophosphatidylcholine
at 4 mM Ca2+
Brassica oleracea var. capitata
3.1.4.4
3
-
lysophosphatidylcholine
at 40 mM Ca2+
Brassica oleracea var. capitata
3.1.4.4
388
-
glycerol
transphosphatidylation
Brassica oleracea var. capitata
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
absolute requirement, optimal at 40 mM
Brassica oleracea var. capitata
Organic Solvent Stability
EC Number
Organic Solvent
Commentary
Organism
3.1.4.4
Glycerol
for measurement of transfer reaction, 2 M included in media to suppress hydrolytic reaction
Brassica oleracea var. capitata
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.4
Brassica oleracea var. capitata
-
var. sabauda
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
lysophosphatidylcholine + glycerol
-
650848
Brassica oleracea var. capitata
?
-
650848
Brassica oleracea var. capitata
?
3.1.4.4
lysophosphatidylcholine + H2O
about 20 times slower than reaction of phosphatidylcholine
650848
Brassica oleracea var. capitata
choline + lysophosphatidate
-
650848
Brassica oleracea var. capitata
?
3.1.4.4
phosphatidylcholine + glycerol
-
650848
Brassica oleracea var. capitata
?
-
650848
Brassica oleracea var. capitata
?
3.1.4.4
phosphatidylcholine + H2O
-
650848
Brassica oleracea var. capitata
choline + phosphatidate
-
650848
Brassica oleracea var. capitata
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
-
-
Brassica oleracea var. capitata
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.4
CTAB
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
Brassica oleracea var. capitata
3.1.4.4
SDS
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
Brassica oleracea var. capitata
3.1.4.4
Triton X-100
inhibitory at concentrations at or above that of 1 lysophosphatidylcholine
Brassica oleracea var. capitata
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.4.4
1
-
lysophosphatidylcholine
at 4 mM Ca2+
Brassica oleracea var. capitata
3.1.4.4
3
-
lysophosphatidylcholine
at 40 mM Ca2+
Brassica oleracea var. capitata
3.1.4.4
388
-
glycerol
transphosphatidylation
Brassica oleracea var. capitata
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.4
Ca2+
absolute requirement, optimal at 40 mM
Brassica oleracea var. capitata
Organic Solvent Stability (protein specific)
EC Number
Organic Solvent
Commentary
Organism
3.1.4.4
Glycerol
for measurement of transfer reaction, 2 M included in media to suppress hydrolytic reaction
Brassica oleracea var. capitata
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.4
lysophosphatidylcholine + glycerol
-
650848
Brassica oleracea var. capitata
?
-
650848
Brassica oleracea var. capitata
?
3.1.4.4
lysophosphatidylcholine + H2O
about 20 times slower than reaction of phosphatidylcholine
650848
Brassica oleracea var. capitata
choline + lysophosphatidate
-
650848
Brassica oleracea var. capitata
?
3.1.4.4
phosphatidylcholine + glycerol
-
650848
Brassica oleracea var. capitata
?
-
650848
Brassica oleracea var. capitata
?
3.1.4.4
phosphatidylcholine + H2O
-
650848
Brassica oleracea var. capitata
choline + phosphatidate
-
650848
Brassica oleracea var. capitata
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.4
5.5
-
-
Brassica oleracea var. capitata