Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Volohonsky, G.; Tuby, C.N.; Porat, N.; Wellman-Rousseau, M.; Visvikis, A.; Leroy, P.; Rashi, S.; Steinberg, P.; Stark, A.A.
    A spectrophotometric assay of gamma-glutamylcysteine synthetase and glutathione synthetase in crude extracts from tissues and cultured mammalian cells (2002), Chem. Biol. Interact., 140, 49-65.
    View publication on PubMed

General Stability

EC Number General Stability Organism
6.3.2.3 glycerol is required for stabilization of the enzyme during freezing Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.3 additional information no inhibition by acivicin Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.3 Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly Rattus norvegicus
-
 ADP + phosphate + glutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.3 Rattus norvegicus
-
 Sprague-Dawley rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.3 from kidney to homogeneity, partially from liver Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.3.2.3 kidney
-
 Rattus norvegicus
-
6.3.2.3 liver
-
 Rattus norvegicus
-
6.3.2.3 M22 cell tumorigenic cell line derived from OC22 by reiterated treatment with N-methyl-N-nitro-N-nitrosoguanidine Rattus norvegicus
-
6.3.2.3 OC/CDE22 cell a non-tumorigenic cell line of oval cells established from livers of rats undergoing carcinogenesis by the choline deficient/ethionine-supplemented diet for 12 weeks Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.3 additional information
-
 assay development, activity under different reaction conditions in rat liver Rattus norvegicus
6.3.2.3 0.0039
-
 cell line OC/CDE22, GSH depleted Rattus norvegicus
6.3.2.3 0.0047
-
 cell line M22 Rattus norvegicus
6.3.2.3 0.0064
-
 cell line OC/CDE22 Rattus norvegicus
6.3.2.3 0.0078
-
 cell line M22, GSH depleted Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
6.3.2.3 -20°C, purified enzyme, 30% glycerol, at least 2 years stable Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.3 ATP + gamma-L-Glu-L-Cys + Gly
-
 Rattus norvegicus ADP + phosphate + glutathione
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.3 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.3 8
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.3 ATP
-
 Rattus norvegicus