Literature summary extracted from

Healy, V.L.; Park, I.S.; Walsh, C.T.
Active-site mutants of the VanC2 D-alanyl-D-serine ligase, characteristic of one vancomycin-resistant bacterial phenotype, revert towards wild-type D-alanyl-D-alanine ligases (1998), Chem. Biol., 5, 197-207.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
6.3.2.4	-	Escherichia coli

Protein Variants

EC Number	Protein Variants	Comment	Organism
6.3.2.4	L282R	11fold decrease in kcat for D-alanine, 18fold increase in Km for D-alanine, 23fold decrease in Km for D-serine, 9fold drop in kcat for D-serine	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
6.3.2.4	1.13	-	D-alanine	-	Escherichia coli
6.3.2.4	56	-	D-serine	-	Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
6.3.2.4	ATP + D-alanine	Escherichia coli	-	ADP + phosphate + D-alanyl-D-alanine	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.3.2.4	Escherichia coli	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
6.3.2.4	recombinant Ddl	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.3.2.4	ATP + D-alanine	-	Escherichia coli	ADP + phosphate + D-alanyl-D-alanine	-	?
6.3.2.4	ATP + D-serine	-	Escherichia coli	ADP + phosphate + D-alanyl-D-serine	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
6.3.2.4	Ddl	-	Escherichia coli

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
6.3.2.4	2.6	-	D-serine	-	Escherichia coli
6.3.2.4	31.2	-	D-alanine	-	Escherichia coli

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Release 2023.1 (January 2023)