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Literature summary extracted from

  • Weber, J.H.; Vishnyakov, A.; Hambach, K.; Schultz, A.; Schultz, J.E.; Linder, J.U.
    Adenylyl cyclases from Plasmodium, Paramecium and Tetrahymena are novel ion channel/enzyme fusion proteins (2003), Cell. Signal., 16, 115-125.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.1 cloning of cDNA Tetrahymena pyriformis
4.6.1.1 cloning of cDNA, expression in Sf9 cells, HEK293 and Xenopus laevis oocytes fails Paramecium tetraurelia
4.6.1.1 cloning of cDNA, expression of synthetic AC gene PfAC526-884 in Sf9 cells Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.1 0.12
-
 ATP recombinant AC expressed from synthetic AC gene PfAC526-884 Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.6.1.1 membrane
-
 Paramecium tetraurelia 16020
-
4.6.1.1 membrane
-
 Tetrahymena pyriformis 16020
-
4.6.1.1 membrane
-
 Plasmodium falciparum 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.1 Mg2+ 2 mM required for activity, recombinant AC expressed from synthetic AC gene PfAC526-884 Plasmodium falciparum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.1 97000
-
 x * 97000, deduced from nucleotide sequence Plasmodium falciparum
4.6.1.1 98000
-
 x * 98000, deduced from nucleotide sequence Paramecium tetraurelia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.1 ATP Paramecium tetraurelia
-
 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP Tetrahymena pyriformis
-
 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP Plasmodium falciparum
-
 3',5'-cyclic AMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.1 Paramecium tetraurelia Q8I759
-
-
4.6.1.1 Plasmodium falciparum Q8I7A1
-
-
4.6.1.1 Tetrahymena pyriformis Q8I0I1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.1 recombinant AC expressed from synthetic AC gene PfAC526-884 Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.1 0.04
-
 recombinant AC expressed from synthetic AC gene PfAC526-884 Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.1 ATP
-
 Paramecium tetraurelia 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP
-
 Tetrahymena pyriformis 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP
-
 Plasmodium falciparum 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP adenylyl cyclase also displays ion channel properties Paramecium tetraurelia 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP adenylyl cyclase also displays ion channel properties Tetrahymena pyriformis 3',5'-cyclic AMP + diphosphate
-
 ?
4.6.1.1 ATP adenylyl cyclase also displays ion channel properties Plasmodium falciparum 3',5'-cyclic AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.1 ? x * 97000, deduced from nucleotide sequence Plasmodium falciparum
4.6.1.1 ? x * 98000, deduced from nucleotide sequence Paramecium tetraurelia

Synonyms

EC Number Synonyms Comment Organism
4.6.1.1 AC
-
 Tetrahymena pyriformis
4.6.1.1 AC
-
 Plasmodium falciparum
4.6.1.1 adenylyl cyclase
-
 Paramecium tetraurelia
4.6.1.1 adenylyl cyclase
-
 Tetrahymena pyriformis
4.6.1.1 ion-channel adenylyl cyclase
-
 Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.6.1.1 30
-
 recombinant AC expressed from synthetic AC gene PfAC526-884 Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.1 8.6
-
 recombinant AC expressed from synthetic AC gene PfAC526-884 Plasmodium falciparum