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Literature summary extracted from

  • Holden, H.M.; Thoden, J.B.; Raushel, F.M.
    Carbamoyl phosphate synthetase: an amazing biochemical odyssey from substrate to product (1999), Cell. Mol. Life Sci., 56, 507-522.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 L-ornithine binds to the enzyme’s large subunit at the interface located between the allosteric and the carbamoyl phosphate domains Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.5.5 X-ray crystallographic analysis, the three active sites on the protein are widely separated from one another Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 additional information IMP binds to enzyme but the actual effect of this ligand is dependent upon temperature and assay conditions Escherichia coli
6.3.5.5 UMP
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.5.5 K+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3- Escherichia coli the product carbamoylphosphate is utilized in the synthesis of arginine and pyrimidine nucleotides and in the urea cycle 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.5.5 2 ATP + L-glutamine + hydrogencarbonate + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3- the product carbamoylphosphate is utilized in the synthesis of arginine and pyrimidine nucleotides and in the urea cycle Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 heterodimer alpha,beta, that readily converts to an tetrameric species: 4 * alpha, beta in presence of positive allosteric effectors, the smaller of two subunits contains 382 amino acid residues and is responsible for the hydrolysis of free ammonia, the larger subunit contains 1073 amino acid residues and provides the binding sites for the two MgATP2 molecules required for the assembly of carbamoyl phosphate, structure and reaction mechanism Escherichia coli