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Literature summary extracted from

  • Dock-Bregeon, A.; Sankaranarayanan, R.; Romby, P.; Caillet, J.; Springer, M.; Rees, B.; Francklyn, C.S.; Ehresmann, C.; Moras, D.
    Transfer RNA-mediated editing in threonyl-tRNA synthetase. The class II solution to the double discrimination problem (2000), Cell, 103, 877-884.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.3 lamdaN-threonine-tRNA ligase complexed with Ser-AMS, X-ray diffraction structure determination at 1.65 A resolution and analysis Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.3 D180A charging of tRNAThr with serine, mutant is no longer able to rapidly deacetylate Ser-tRNAThr Escherichia coli
6.1.1.3 H73A/H77A charging of tRNAThr with serine, mutant is no longer able to deacetylate Ser-tRNAThr Escherichia coli
6.1.1.3 additional information truncated lamdaN-enzyme mutant, lacking the N-terminal domains N1 and N2, produces Ser-tRNAThr, reduced activity and altered substrate recognition compared to the wild-type which does nearly not incorporate serine Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.3 Zn2+ used to discriminate against the isosteric valine at the activation step Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr Escherichia coli
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Escherichia coli
-
 class II enzyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.3 ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr functional mechanism and substrate recognition, editing model, 2 separate active sites for substrate binding, binding of tRNA is more specific than the binding of the amino acid Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-serine + tRNAThr very low activity with the wild-type enzyme Escherichia coli AMP + diphosphate + L-seryl-tRNAThr
-
 ?
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Escherichia coli AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.3 Synthetase, threonyl-transfer ribonucleate
-
 Escherichia coli
6.1.1.3 Threonine translase
-
 Escherichia coli
6.1.1.3 Threonine--tRNA ligase
-
 Escherichia coli
6.1.1.3 Threonine-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-ribonucleic synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-tRNA synthetase
-
 Escherichia coli
6.1.1.3 ThrRS
-
 Escherichia coli
6.1.1.3 TRS
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.3 ATP
-
 Escherichia coli