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Literature summary extracted from

  • Humerickhouse, R.; Lohrbach, K.; Li, L.; Bosron, W.F.; Dolan, M.E.
    Characterization of CPT-11 hydrolysis by human liver carboxylesterase isoforms hCE-1 and hCE-2 (2000), Cancer Res., 60, 1189-1192.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.1 0.00034
-
 7-ethyl-10[4-(1-piperidino)-1-piperdino]carbonyloxy-camptothecin isozyme CE-2 Homo sapiens
3.1.1.1 0.0043
-
 7-ethyl-10[4-(1-piperidino)-1-piperdino]carbonyloxy-camptothecin isozyme CE-1 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.1 7-ethyl-10[4-(1-piperidino)-1-piperdino]carbonyloxy-camptothecin + H2O Homo sapiens isozyme CE-2 is responsible for activation of the prodrug 7-ethyl-10-hydroxy-camptothecin + ? i.e. SN-38 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Homo sapiens
-
 isozymes CE-1 and CE-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 isozymes CE-1 and CE-2 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 liver isozymes CE-1 and CE-2 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.1 additional information
-
 isozyme CE-2 shows 5fold higher activity with substrate 7-ethyl-10[4-(1-piperidino)-1-piperdino]carbonyloxy-camptothecin than isozyme CE-1 Homo sapiens
3.1.1.1 40 140 isozyme CE-2 Homo sapiens
3.1.1.1 610
-
 isozyme CE-1 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 4-methylumbelliferyl acetate + H2O
-
 Homo sapiens 4-methylumbelliferol + acetate
-
 ?
3.1.1.1 7-ethyl-10[4-(1-piperidino)-1-piperdino]carbonyloxy-camptothecin + H2O activation of the prodrug, i.e. CPT-11 or irinotecan, a commercial topoisomerase I inhibitor commonly used for treatment of colorectal tumors Homo sapiens 7-ethyl-10-hydroxy-camptothecin + ? i.e. SN-38 ?
3.1.1.1 7-ethyl-10[4-(1-piperidino)-1-piperdino]carbonyloxy-camptothecin + H2O isozyme CE-2 is responsible for activation of the prodrug Homo sapiens 7-ethyl-10-hydroxy-camptothecin + ? i.e. SN-38 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 CE
-
 Homo sapiens