Literature summary extracted from
Li, T.; Juteau, P.; Beaudet, R.; Lepine, F.; Villemur, R.; Bisaillon, J.G.
Purification and characterization of a 4-hydroxybenzoate decarboxylase from an anaerobic coculture (2000), Can. J. Microbiol., 46, 856-859.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
4.1.1.61 |
additional information |
no stimulation by ATP up to 5 mM or phenylphosphate |
uncultured bacterium |
|
General Stability
EC Number |
General Stability |
Organism |
---|
4.1.1.61 |
oxygen sensitive, loss of 50% of activity after 1 h in presence of air at 4°C compared to no activity lost after 7 days under anaerobic conditions at 4°C |
uncultured bacterium |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.1.1.61 |
EDTA |
47% inhibition at 5 mM, Mg2+, Mn2+, Zn2+ or Ca2+ protect, Fe2+ does not |
uncultured bacterium |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.1.1.61 |
5.4 |
- |
4-hydroxybenzoate |
pH 6.0, 37°C |
uncultured bacterium |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.61 |
uncultured bacterium |
- |
Clostridium-like strain 6 |
- |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.1.1.61 |
766 |
- |
- |
uncultured bacterium |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.61 |
3,4-dihydroxybenzoate |
slow reaction |
uncultured bacterium |
benzene-1,2-diol + CO2 |
- |
? |
|
4.1.1.61 |
4-Hydroxybenzoate |
- |
uncultured bacterium |
Phenol + CO2 |
- |
? |
|
4.1.1.61 |
additional information |
no substrates are 2-hydroxybenzoate, 3-hydroxybenzoate, 2,3-dihydroxybenzoate, 2,4-dihydroxybenzoate, 2,5-dihydroxybenzoate, 2,3,4-trihydroxybenzoate, 3,4,5-trihydroxybenzoate |
uncultured bacterium |
? |
- |
? |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.1.1.61 |
5 |
6.5 |
broad |
uncultured bacterium |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
4.1.1.61 |
uncultured bacterium |
isoeolctric focusing |
5.9 |
5.6 |