BRENDA - Enzyme Database

The enzymatic activity of phosphoglycerate mutase from gram-positive endospore-forming bacteria requires Mn2+ and is pH sensitive

Chander, M.; Setlow, B.; Setlow, P.; Can. J. Microbiol. 44, 759-767 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
Sodium metavanadate
0.01 mM and 0.1 mM, 50% and 90% inhibition of phosphoglycerate mutase activity, respectively
Streptomyces coelicolor
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
3.6
-
3-phospho-D-glycerate
25C, pH 6.0
Bacillus subtilis
5.4.2.12
4.2
-
3-phospho-D-glycerate
25C, pH 7.0
Clostridium perfringens
5.4.2.12
4.4
-
3-phospho-D-glycerate
25C, pH 7.0
Bacillus subtilis
5.4.2.12
4.9
-
3-phospho-D-glycerate
25C, pH 8.0
Bacillus subtilis
5.4.2.12
4.9
-
3-phospho-D-glycerate
25C, pH 8.0
Clostridium perfringens
5.4.2.12
5.2
-
3-phospho-D-glycerate
25C, pH 6.0
Clostridium perfringens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Ca2+
1 mm, 19% of activity with 0.5 mM Mn2+
Staphylococcus saprophyticus
5.4.2.12
Co2+
required for activity, 2fold higher activity at 0.4 mM than with 0.5 mM Mn2+
Clostridium perfringens
5.4.2.12
Mn2+
required for activity
Bacillus subtilis
5.4.2.12
Mn2+
required for activity
Clostridium perfringens
5.4.2.12
Mn2+
required for activity
Planococcus citreus
5.4.2.12
Mn2+
required for activity
Sporosarcina ureae
5.4.2.12
Mn2+
required for activity
Staphylococcus saprophyticus
5.4.2.12
additional information
no metal ions required for activity
Micrococcus luteus
5.4.2.12
additional information
no metal ions required for activity
Streptomyces coelicolor
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Bacillus subtilis
-
3-phospho-D-glycerate
-
Bacillus subtilis
r
5.4.2.12
2-phospho-D-glycerate
Micrococcus luteus
-
3-phospho-D-glycerate
-
Micrococcus luteus
r
5.4.2.12
2-phospho-D-glycerate
Streptomyces coelicolor
-
3-phospho-D-glycerate
-
Streptomyces coelicolor
r
5.4.2.12
2-phospho-D-glycerate
Clostridium perfringens
-
3-phospho-D-glycerate
-
Clostridium perfringens
r
5.4.2.12
2-phospho-D-glycerate
Staphylococcus saprophyticus
-
3-phospho-D-glycerate
-
Staphylococcus saprophyticus
r
5.4.2.12
2-phospho-D-glycerate
Sporosarcina ureae
-
3-phospho-D-glycerate
-
Sporosarcina ureae
r
5.4.2.12
2-phospho-D-glycerate
Planococcus citreus
-
3-phospho-D-glycerate
-
Planococcus citreus
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Bacillus subtilis
-
-
-
5.4.2.12
Clostridium perfringens
-
-
-
5.4.2.12
Micrococcus luteus
-
-
-
5.4.2.12
Planococcus citreus
-
-
-
5.4.2.12
Sporosarcina ureae
-
-
-
5.4.2.12
Staphylococcus saprophyticus
-
-
-
5.4.2.12
Streptomyces coelicolor
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
partialy purified
Bacillus subtilis
5.4.2.12
partialy purified
Clostridium perfringens
5.4.2.12
partialy purified
Micrococcus luteus
5.4.2.12
partialy purified
Planococcus citreus
5.4.2.12
partialy purified
Sporosarcina ureae
5.4.2.12
partialy purified
Staphylococcus saprophyticus
5.4.2.12
partialy purified
Streptomyces coelicolor
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.4.2.12
0.003
-
-
Sporosarcina ureae
5.4.2.12
0.014
-
-
Bacillus subtilis
5.4.2.12
0.1
-
-
Staphylococcus saprophyticus
5.4.2.12
0.24
-
-
Clostridium perfringens
5.4.2.12
0.49
-
-
Micrococcus luteus
5.4.2.12
0.93
-
-
Streptomyces coelicolor
5.4.2.12
1
-
-
Planococcus citreus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
650753
Bacillus subtilis
3-phospho-D-glycerate
-
650753
Bacillus subtilis
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Micrococcus luteus
3-phospho-D-glycerate
-
650753
Micrococcus luteus
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Streptomyces coelicolor
3-phospho-D-glycerate
-
650753
Streptomyces coelicolor
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Clostridium perfringens
3-phospho-D-glycerate
-
650753
Clostridium perfringens
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Staphylococcus saprophyticus
3-phospho-D-glycerate
-
650753
Staphylococcus saprophyticus
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Sporosarcina ureae
3-phospho-D-glycerate
-
650753
Sporosarcina ureae
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Planococcus citreus
3-phospho-D-glycerate
-
650753
Planococcus citreus
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
8
-
-
Bacillus subtilis
5.4.2.12
8
-
-
Clostridium perfringens
5.4.2.12
8
-
-
Micrococcus luteus
5.4.2.12
8
-
-
Planococcus citreus
5.4.2.12
8
-
-
Sporosarcina ureae
5.4.2.12
8
-
-
Staphylococcus saprophyticus
5.4.2.12
8
-
-
Streptomyces coelicolor
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.2.12
6
8
24% and 36% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively
Bacillus subtilis
5.4.2.12
6
8
7.3% and 39% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively, inhibition at low pH is reversed by elevating the pH
Clostridium perfringens
5.4.2.12
6
8
little change in activity between pH 6.0 and pH 8.0
Micrococcus luteus
5.4.2.12
6
8
1.7% and 19% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively
Planococcus citreus
5.4.2.12
6
8
1% and 18% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively
Sporosarcina ureae
5.4.2.12
6
8
3.1% and 21% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively
Staphylococcus saprophyticus
5.4.2.12
6
8
little change in activity between pH 6.0 and pH 8.0
Streptomyces coelicolor
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Bacillus subtilis
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Clostridium perfringens
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Micrococcus luteus
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Planococcus citreus
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Sporosarcina ureae
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Staphylococcus saprophyticus
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Streptomyces coelicolor
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Bacillus subtilis
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Clostridium perfringens
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Micrococcus luteus
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Planococcus citreus
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Sporosarcina ureae
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Staphylococcus saprophyticus
5.4.2.12
additional information
2,3-bisphosphoglycerate independent phosphoglycerate mutase
Streptomyces coelicolor
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.2.12
Sodium metavanadate
0.01 mM and 0.1 mM, 50% and 90% inhibition of phosphoglycerate mutase activity, respectively
Streptomyces coelicolor
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.2.12
3.6
-
3-phospho-D-glycerate
25C, pH 6.0
Bacillus subtilis
5.4.2.12
4.2
-
3-phospho-D-glycerate
25C, pH 7.0
Clostridium perfringens
5.4.2.12
4.4
-
3-phospho-D-glycerate
25C, pH 7.0
Bacillus subtilis
5.4.2.12
4.9
-
3-phospho-D-glycerate
25C, pH 8.0
Bacillus subtilis
5.4.2.12
4.9
-
3-phospho-D-glycerate
25C, pH 8.0
Clostridium perfringens
5.4.2.12
5.2
-
3-phospho-D-glycerate
25C, pH 6.0
Clostridium perfringens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.2.12
Ca2+
1 mm, 19% of activity with 0.5 mM Mn2+
Staphylococcus saprophyticus
5.4.2.12
Co2+
required for activity, 2fold higher activity at 0.4 mM than with 0.5 mM Mn2+
Clostridium perfringens
5.4.2.12
Mn2+
required for activity
Bacillus subtilis
5.4.2.12
Mn2+
required for activity
Clostridium perfringens
5.4.2.12
Mn2+
required for activity
Planococcus citreus
5.4.2.12
Mn2+
required for activity
Sporosarcina ureae
5.4.2.12
Mn2+
required for activity
Staphylococcus saprophyticus
5.4.2.12
additional information
no metal ions required for activity
Micrococcus luteus
5.4.2.12
additional information
no metal ions required for activity
Streptomyces coelicolor
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Bacillus subtilis
-
3-phospho-D-glycerate
-
Bacillus subtilis
r
5.4.2.12
2-phospho-D-glycerate
Micrococcus luteus
-
3-phospho-D-glycerate
-
Micrococcus luteus
r
5.4.2.12
2-phospho-D-glycerate
Streptomyces coelicolor
-
3-phospho-D-glycerate
-
Streptomyces coelicolor
r
5.4.2.12
2-phospho-D-glycerate
Clostridium perfringens
-
3-phospho-D-glycerate
-
Clostridium perfringens
r
5.4.2.12
2-phospho-D-glycerate
Staphylococcus saprophyticus
-
3-phospho-D-glycerate
-
Staphylococcus saprophyticus
r
5.4.2.12
2-phospho-D-glycerate
Sporosarcina ureae
-
3-phospho-D-glycerate
-
Sporosarcina ureae
r
5.4.2.12
2-phospho-D-glycerate
Planococcus citreus
-
3-phospho-D-glycerate
-
Planococcus citreus
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
partialy purified
Bacillus subtilis
5.4.2.12
partialy purified
Clostridium perfringens
5.4.2.12
partialy purified
Micrococcus luteus
5.4.2.12
partialy purified
Planococcus citreus
5.4.2.12
partialy purified
Sporosarcina ureae
5.4.2.12
partialy purified
Staphylococcus saprophyticus
5.4.2.12
partialy purified
Streptomyces coelicolor
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
5.4.2.12
0.003
-
-
Sporosarcina ureae
5.4.2.12
0.014
-
-
Bacillus subtilis
5.4.2.12
0.1
-
-
Staphylococcus saprophyticus
5.4.2.12
0.24
-
-
Clostridium perfringens
5.4.2.12
0.49
-
-
Micrococcus luteus
5.4.2.12
0.93
-
-
Streptomyces coelicolor
5.4.2.12
1
-
-
Planococcus citreus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
650753
Bacillus subtilis
3-phospho-D-glycerate
-
650753
Bacillus subtilis
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Micrococcus luteus
3-phospho-D-glycerate
-
650753
Micrococcus luteus
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Streptomyces coelicolor
3-phospho-D-glycerate
-
650753
Streptomyces coelicolor
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Clostridium perfringens
3-phospho-D-glycerate
-
650753
Clostridium perfringens
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Staphylococcus saprophyticus
3-phospho-D-glycerate
-
650753
Staphylococcus saprophyticus
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Sporosarcina ureae
3-phospho-D-glycerate
-
650753
Sporosarcina ureae
r
5.4.2.12
2-phospho-D-glycerate
-
650753
Planococcus citreus
3-phospho-D-glycerate
-
650753
Planococcus citreus
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.2.12
8
-
-
Bacillus subtilis
5.4.2.12
8
-
-
Clostridium perfringens
5.4.2.12
8
-
-
Micrococcus luteus
5.4.2.12
8
-
-
Planococcus citreus
5.4.2.12
8
-
-
Sporosarcina ureae
5.4.2.12
8
-
-
Staphylococcus saprophyticus
5.4.2.12
8
-
-
Streptomyces coelicolor
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
5.4.2.12
6
8
24% and 36% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively
Bacillus subtilis
5.4.2.12
6
8
7.3% and 39% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively, inhibition at low pH is reversed by elevating the pH
Clostridium perfringens
5.4.2.12
6
8
little change in activity between pH 6.0 and pH 8.0
Micrococcus luteus
5.4.2.12
6
8
1.7% and 19% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively
Planococcus citreus
5.4.2.12
6
8
1% and 18% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively
Sporosarcina ureae
5.4.2.12
6
8
3.1% and 21% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively
Staphylococcus saprophyticus
5.4.2.12
6
8
little change in activity between pH 6.0 and pH 8.0
Streptomyces coelicolor