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Literature summary extracted from
- The enzymatic activity of phosphoglycerate mutase from gram-positive endospore-forming bacteria requires Mn2+ and is pH sensitive (1998), Can. J. Microbiol., 44, 759-767.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.12 | Sodium metavanadate | 0.01 mM and 0.1 mM, 50% and 90% inhibition of phosphoglycerate mutase activity, respectively | Streptomyces coelicolor |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.2.12 | 3.6 | - |
3-phospho-D-glycerate | 25°C, pH 6.0 | Bacillus subtilis | |
| 5.4.2.12 | 4.2 | - |
3-phospho-D-glycerate | 25°C, pH 7.0 | Clostridium perfringens | |
| 5.4.2.12 | 4.4 | - |
3-phospho-D-glycerate | 25°C, pH 7.0 | Bacillus subtilis | |
| 5.4.2.12 | 4.9 | - |
3-phospho-D-glycerate | 25°C, pH 8.0 | Bacillus subtilis | |
| 5.4.2.12 | 4.9 | - |
3-phospho-D-glycerate | 25°C, pH 8.0 | Clostridium perfringens | |
| 5.4.2.12 | 5.2 | - |
3-phospho-D-glycerate | 25°C, pH 6.0 | Clostridium perfringens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.12 | Ca2+ | 1 mm, 19% of activity with 0.5 mM Mn2+ | Staphylococcus saprophyticus | |
| 5.4.2.12 | Co2+ | required for activity, 2fold higher activity at 0.4 mM than with 0.5 mM Mn2+ | Clostridium perfringens | |
| 5.4.2.12 | Mn2+ | required for activity | Bacillus subtilis | |
| 5.4.2.12 | Mn2+ | required for activity | Clostridium perfringens | |
| 5.4.2.12 | Mn2+ | required for activity | Staphylococcus saprophyticus | |
| 5.4.2.12 | Mn2+ | required for activity | Sporosarcina ureae | |
| 5.4.2.12 | Mn2+ | required for activity | Planococcus citreus | |
| 5.4.2.12 | additional information | no metal ions required for activity | Micrococcus luteus | |
| 5.4.2.12 | additional information | no metal ions required for activity | Streptomyces coelicolor |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.12 | 2-phospho-D-glycerate | Bacillus subtilis | - |
3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | Micrococcus luteus | - |
3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | Streptomyces coelicolor | - |
3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | Clostridium perfringens | - |
3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | Staphylococcus saprophyticus | - |
3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | Sporosarcina ureae | - |
3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | Planococcus citreus | - |
3-phospho-D-glycerate | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.2.12 | Bacillus subtilis | - |
- |
- |
| 5.4.2.12 | Clostridium perfringens | - |
- |
- |
| 5.4.2.12 | Micrococcus luteus | - |
- |
- |
| 5.4.2.12 | Planococcus citreus | - |
- |
- |
| 5.4.2.12 | Sporosarcina ureae | - |
- |
- |
| 5.4.2.12 | Staphylococcus saprophyticus | - |
- |
- |
| 5.4.2.12 | Streptomyces coelicolor | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.2.12 | partialy purified | Bacillus subtilis |
| 5.4.2.12 | partialy purified | Micrococcus luteus |
| 5.4.2.12 | partialy purified | Streptomyces coelicolor |
| 5.4.2.12 | partialy purified | Clostridium perfringens |
| 5.4.2.12 | partialy purified | Staphylococcus saprophyticus |
| 5.4.2.12 | partialy purified | Sporosarcina ureae |
| 5.4.2.12 | partialy purified | Planococcus citreus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.12 | 0.003 | - |
- |
Sporosarcina ureae |
| 5.4.2.12 | 0.014 | - |
- |
Bacillus subtilis |
| 5.4.2.12 | 0.1 | - |
- |
Staphylococcus saprophyticus |
| 5.4.2.12 | 0.24 | - |
- |
Clostridium perfringens |
| 5.4.2.12 | 0.49 | - |
- |
Micrococcus luteus |
| 5.4.2.12 | 0.93 | - |
- |
Streptomyces coelicolor |
| 5.4.2.12 | 1 | - |
- |
Planococcus citreus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.2.12 | 2-phospho-D-glycerate | - |
Bacillus subtilis | 3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | - |
Micrococcus luteus | 3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | - |
Streptomyces coelicolor | 3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | - |
Clostridium perfringens | 3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | - |
Staphylococcus saprophyticus | 3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | - |
Sporosarcina ureae | 3-phospho-D-glycerate | - |
r | |
| 5.4.2.12 | 2-phospho-D-glycerate | - |
Planococcus citreus | 3-phospho-D-glycerate | - |
r | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.2.12 | PGM | - |
Bacillus subtilis |
| 5.4.2.12 | PGM | - |
Micrococcus luteus |
| 5.4.2.12 | PGM | - |
Streptomyces coelicolor |
| 5.4.2.12 | PGM | - |
Clostridium perfringens |
| 5.4.2.12 | PGM | - |
Staphylococcus saprophyticus |
| 5.4.2.12 | PGM | - |
Sporosarcina ureae |
| 5.4.2.12 | PGM | - |
Planococcus citreus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.12 | 8 | - |
- |
Bacillus subtilis |
| 5.4.2.12 | 8 | - |
- |
Micrococcus luteus |
| 5.4.2.12 | 8 | - |
- |
Streptomyces coelicolor |
| 5.4.2.12 | 8 | - |
- |
Clostridium perfringens |
| 5.4.2.12 | 8 | - |
- |
Staphylococcus saprophyticus |
| 5.4.2.12 | 8 | - |
- |
Sporosarcina ureae |
| 5.4.2.12 | 8 | - |
- |
Planococcus citreus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.2.12 | 6 | 8 | 1% and 18% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively | Sporosarcina ureae |
| 5.4.2.12 | 6 | 8 | 1.7% and 19% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively | Planococcus citreus |
| 5.4.2.12 | 6 | 8 | 24% and 36% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively | Bacillus subtilis |
| 5.4.2.12 | 6 | 8 | 3.1% and 21% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively | Staphylococcus saprophyticus |
| 5.4.2.12 | 6 | 8 | 7.3% and 39% of maximal activity at pH 6.0 and pH 7.0 in the presence of 1 mM and 0.5 mM Mn2+, respectively, inhibition at low pH is reversed by elevating the pH | Clostridium perfringens |
| 5.4.2.12 | 6 | 8 | little change in activity between pH 6.0 and pH 8.0 | Micrococcus luteus |
| 5.4.2.12 | 6 | 8 | little change in activity between pH 6.0 and pH 8.0 | Streptomyces coelicolor |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.2.12 | additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Bacillus subtilis | |
| 5.4.2.12 | additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Micrococcus luteus | |
| 5.4.2.12 | additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Streptomyces coelicolor | |
| 5.4.2.12 | additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Clostridium perfringens | |
| 5.4.2.12 | additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Staphylococcus saprophyticus | |
| 5.4.2.12 | additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Sporosarcina ureae | |
| 5.4.2.12 | additional information | 2,3-bisphosphoglycerate independent phosphoglycerate mutase | Planococcus citreus |
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