Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • La, I.J.; Kim, H.J.; Yoon, M.Y.
    Kinetic mechanism in the absence of metal of Hafnia alvei aspartase in the amination direction (2001), Bull. Korean Chem. Soc., 22, 288-292.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.1.1 ammonium
-
Hafnia alvei
4.3.1.1 fumarate
-
Hafnia alvei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.1.1 0.2
-
fumarate 25°C, pH 7.0, in presence of Mg2+ Hafnia alvei
4.3.1.1 0.58
-
aspartate 25°C, pH 7.0, in presence of Mg2+ Hafnia alvei
4.3.1.1 0.76
-
fumarate 25°C, pH 7.0, in absence of Mg2+ Hafnia alvei
4.3.1.1 1.73
-
aspartate 25°C, pH 7.0, in absence of Mg2+ Hafnia alvei

Organism

EC Number Organism UniProt Comment Textmining
4.3.1.1 Hafnia alvei
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.3.1.1 L-aspartate = fumarate + NH3 mechanism, kinetics Hafnia alvei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.1.1 additional information
-
kinetic analyses Hafnia alvei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.1.1 fumarate + NH3
-
Hafnia alvei L-aspartate
-
?
4.3.1.1 L-aspartate
-
Hafnia alvei fumarate + NH3
-
r

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.1.1 0.165
-
fumarate 25°C, pH 7.0, in presence of Mg2+ Hafnia alvei
4.3.1.1 1.19
-
ammonium 25°C, pH 7.0, in absence of Mg2+ Hafnia alvei
4.3.1.1 2.2
-
ammonium 25°C, pH 7.0, in presence of Mg2+ Hafnia alvei
4.3.1.1 2.83
-
fumarate 25°C, pH 7.0, in absence of Mg2+ Hafnia alvei