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Literature summary extracted from

  • Morales, A.C.; Nozawa, S.R.; Thedei, G.Jr.; Maccheroni, W.Jr.; Rossi, A.
    Properties of a constitutive alkaline phosphatase from strain 74A of the mold Neurospora crassa (2000), Braz. J. Med. Biol. Res., 33, 905-912.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.1 EDTA
-
 Neurospora crassa
3.1.3.1 histidine
-
 Neurospora crassa
3.1.3.1 Tris-HCl buffer
-
 Neurospora crassa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.1 0.35
-
 4-nitrophenyl phosphate pH 8.9, 37°C Neurospora crassa
3.1.3.1 0.44
-
 Alpha-naphthyl phosphate pH 8.9, 37°C Neurospora crassa
3.1.3.1 0.47
-
 L-histidinyl phosphate pH 8.9, 37°C Neurospora crassa
3.1.3.1 2.46
-
 beta-glyceryl phosphate pH 8.9, 37°C Neurospora crassa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 Be2+
-
 Neurospora crassa
3.1.3.1 Mg2+ activation Neurospora crassa
3.1.3.1 Zn2+ activation Neurospora crassa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 56000
-
 1 * 56000, SDS-PAGE Neurospora crassa
3.1.3.1 58000
-
 gel filtration Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Neurospora crassa
-
 constitutive enzyme expression
-
3.1.3.1 Neurospora crassa St. L. 74A
-
 constitutive enzyme expression
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 to homogeneity, 131.4fold Neurospora crassa

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.1 a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Neurospora crassa
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.1 culture condition:acetate-grown cell most efficient in presence of saturating phosphate at pH 7.1 Neurospora crassa
-
3.1.3.1 mycelium
-
 Neurospora crassa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.1 223.3
-
 purified enzyme Neurospora crassa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Neurospora crassa 4-nitrophenol + phosphate
-
 ?
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Neurospora crassa St. L. 74A 4-nitrophenol + phosphate
-
 ?
3.1.3.1 alpha-naphthyl phosphate + H2O
-
 Neurospora crassa alpha-naphthol + phosphate
-
 ?
3.1.3.1 alpha-naphthyl phosphate + H2O
-
 Neurospora crassa St. L. 74A alpha-naphthol + phosphate
-
 ?
3.1.3.1 beta-glyceryl phosphate + H2O
-
 Neurospora crassa glycerol + phosphate
-
 ?
3.1.3.1 beta-glyceryl phosphate + H2O
-
 Neurospora crassa St. L. 74A glycerol + phosphate
-
 ?
3.1.3.1 L-histidinyl phosphate + H2O
-
 Neurospora crassa L-histidinol + phosphate
-
 ?
3.1.3.1 L-histidinyl phosphate + H2O
-
 Neurospora crassa St. L. 74A L-histidinol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 monomer 1 * 56000, SDS-PAGE Neurospora crassa

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Neurospora crassa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 additional information
-
 Tris-HCl partly protects against heat inactivation Neurospora crassa
3.1.3.1 70
-
 half-life 19 min in Tris-HCl, 2.3 min in CHES Neurospora crassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 9.5 10.5
-
 Neurospora crassa