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Literature summary extracted from
- Continuous production of (R)-phenylacetylcarbinol in an enzyme-membrane reactor using a potent mutant of pyruvate decarboxylase from Zymomonas mobilis (2001), Biotechnol. Bioeng., 74, 317-325.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.1.1.1 | synthesis | synthesis of (R)-phenylacetylcarbinol from cheap substrates in an aqueous reaction system by W392M mutant PDC, alternative strategy to the current fermentative process free of any unwanted by-product | Zymomonas mobilis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.1.1.1 | expression of wild-type and W392M mutant PDC in Escherichia coli K12 | Zymomonas mobilis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.1.1 | W392M | higher carboligase/(R)-phenylacetylcarbinol-producing activity, more stable and higher resistance towards acetaldehyde than wild-type PDC | Zymomonas mobilis |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.1.1.1 | low stability in the isolated state, less stable than PDC from Zymomonas mobilis | Saccharomyces cerevisiae |
| 4.1.1.1 | more stable than PDC from Saccharomyces cerevisiae, stirring deactivates significantly | Zymomonas mobilis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.1.1 | acetaldehyde | inhibits, more resistant than PDC from Zymomonas mobilis, 8 mM, 2h, stable | Saccharomyces cerevisiae |  |
| 4.1.1.1 | acetaldehyde | 0.4 mM, inactivates | Zymomonas mobilis | |
| 4.1.1.1 | benzaldehyde | inhibits formation of (R)-1-phenyl-1-hydroxy-propane-2-one | Zymomonas mobilis | |
| 4.1.1.1 | additional information | no product inhibition by (R)-1-phenyl-1-hydroxy-propane-2-one | Zymomonas mobilis | |
| 4.1.1.1 | PO43- | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.1 | additional information | - |
additional information | kinetic data, kinetic model | Zymomonas mobilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.1 | Saccharomyces cerevisiae | - |
- |
- |
| 4.1.1.1 | Zymomonas mobilis | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 | catalyzes the carboligation of 2 aldehydes as a side reaction, mechanisms of both reactions | Saccharomyces cerevisiae | |
| 4.1.1.1 | a 2-oxo carboxylate = an aldehyde + CO2 | catalyzes the carboligation of 2 aldehydes as a side reaction, mechanisms of both reactions, thermodynamic data | Zymomonas mobilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.1 | acetaldehyde + acetaldehyde | carboligation of 2 aldehydes as a side reaction of PDC | Saccharomyces cerevisiae | acetoin | - |
? | |
| 4.1.1.1 | acetaldehyde + acetaldehyde | carboligation of 2 aldehydes as a side reaction of PDC | Zymomonas mobilis | acetoin | - |
? | |
| 4.1.1.1 | acetaldehyde + benzaldehyde | carboligation of 2 aldehydes as a side reaction of PDC, high carboligase activity, more active than PDC from Zymomonas mobilis | Saccharomyces cerevisiae | (R)-1-phenyl-1-hydroxy-propane-2-one | (R)-phenylacetylcarbinol | ? | |
| 4.1.1.1 | acetaldehyde + benzaldehyde | carboligation of 2 aldehydes as a side reaction of PDC, less active than PDC from Saccharomyces cerevisiae | Zymomonas mobilis | (R)-1-phenyl-1-hydroxy-propane-2-one | (R)-phenylacetylcarbinol | ? | |
| 4.1.1.1 | pyruvate | nonoxidative decarboxylation, main reaction | Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
| 4.1.1.1 | pyruvate | nonoxidative decarboxylation, main reaction | Zymomonas mobilis | acetaldehyde + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.1 | PDCS.c. | - |
Saccharomyces cerevisiae |
| 4.1.1.1 | PDCZ.m. | - |
Zymomonas mobilis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 25 | - |
assay at | Saccharomyces cerevisiae |
| 4.1.1.1 | 25 | - |
assay at | Zymomonas mobilis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 20 | 30 | only slightly differing catalytic activity in the range, +/-10% relative to 25°C | Zymomonas mobilis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 25 | - |
rapidly inactivated at | Saccharomyces cerevisiae |
| 4.1.1.1 | 30 | - |
rapidly inactivated at | Saccharomyces cerevisiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.1.1 | 6.5 | - |
assay at | Saccharomyces cerevisiae |
| 4.1.1.1 | 6.5 | 7 | carboligation of acetaldehyde and benzaldehyde, optimal activity in potassium phosphate buffer | Zymomonas mobilis |
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