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Literature summary extracted from
- Immobilization of tannase by microencapsulation and its kinetic characteristics (2003), Biotechnol. Appl. Biochem., 40, 151-155.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.1.20 | biotechnology | immobilization of the enzyme by microencapsulation with a coacervate calcium alginate membrane surrounding a liquid core improves the thermal and pH stability significantly | Aspergillus niger |
| 3.1.1.20 | biotechnology | immobilization of enzyme by microencapsulation with a coacervate calcium alginate membrane surrounding a liquid core. Yield is 36.8% of initial enzyme activity, pH stability and thermal stability improve after microencapsulation. Enzyme can be used for up to 15 runs | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.20 | additional information | - |
additional information | kinetics | Aspergillus niger | |
| 3.1.1.20 | 0.000011 | - |
Tannic acid | free enzyme, pH 5.0, 30°C | Aspergillus niger |  |
| 3.1.1.20 | 0.000041 | - |
Tannic acid | immobilized enzyme, pH 6.0, 40°C | Aspergillus niger | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 310000 | - |
- |
Aspergillus niger |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | additional information | Aspergillus niger | the enzyme hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters | ? | - |
? | |
| 3.1.1.20 | tannic acid + H2O | Aspergillus niger | - |
10 gallate + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.20 | Aspergillus niger | - |
- |
- |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 380 | - |
- |
Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.20 | digallate + H2O | - |
Aspergillus niger | gallate | - |
? | |
| 3.1.1.20 | additional information | the enzyme hydrolyzes the ester and depside bonds of gallotannins and gallic acid esters | Aspergillus niger | ? | - |
? | |
| 3.1.1.20 | tannic acid + H2O | - |
Aspergillus niger | 10 gallate + D-glucose | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 30 | - |
immobilized enzyme | Aspergillus niger |
| 3.1.1.20 | 30 | - |
free enzyme | Aspergillus niger |
| 3.1.1.20 | 40 | - |
immobilized enzyme | Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 20 | 60 | immobilized enzyme, 87% remaining activity after 1 h at 60°C | Aspergillus niger |
| 3.1.1.20 | 30 | 45 | immobilized enzyme, 30% remaining activity after 1 h at 60°C | Aspergillus niger |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.20 | 5 | - |
free enzyme | Aspergillus niger |
| 3.1.1.20 | 6 | - |
immobilized enzyme | Aspergillus niger |
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Release 2023.1 (January 2023)
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