BRENDA - Enzyme Database

Purification of native enolase from medically important Candida species

Ballantyne, D.S.; Warmington, J.R.; Biotechnol. Appl. Biochem. 31, 213-218 (2000)
No PubMed abstract available

Data extracted from this reference:

Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Candida albicans
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Candida glabrata
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Candida parapsilosis
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Clavispora lusitaniae
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Pichia kudriavzevii
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Candida albicans
-
-
-
4.2.1.11
Candida glabrata
-
-
-
4.2.1.11
Candida parapsilosis
-
-
-
4.2.1.11
Clavispora lusitaniae
-
-
-
4.2.1.11
Pichia kudriavzevii
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
using anion- and cation exchange chromatography
Candida albicans
4.2.1.11
using anion- and cation exchange chromatography
Candida glabrata
4.2.1.11
using anion- and cation exchange chromatography
Candida parapsilosis
4.2.1.11
using anion- and cation exchange chromatography
Clavispora lusitaniae
4.2.1.11
using anion- and cation exchange chromatography
Pichia kudriavzevii
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
60
70
pH 7.4
Candida albicans
4.2.1.11
60
70
pH 7.4
Candida glabrata
4.2.1.11
60
70
pH 7.4
Candida parapsilosis
4.2.1.11
60
70
pH 7.4
Clavispora lusitaniae
4.2.1.11
60
70
pH 7.4
Pichia kudriavzevii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
650658
Pichia kudriavzevii
phosphoenolpyruvate
-
650658
Pichia kudriavzevii
r
4.2.1.11
2-phospho-D-glycerate
-
650658
Candida albicans
phosphoenolpyruvate
-
650658
Candida albicans
r
4.2.1.11
2-phospho-D-glycerate
-
650658
Candida parapsilosis
phosphoenolpyruvate
-
650658
Candida parapsilosis
r
4.2.1.11
2-phospho-D-glycerate
-
650658
Candida glabrata
phosphoenolpyruvate
-
650658
Candida glabrata
r
4.2.1.11
2-phospho-D-glycerate
-
650658
Clavispora lusitaniae
phosphoenolpyruvate
-
650658
Clavispora lusitaniae
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47000, SDS-PAGE
Candida albicans
4.2.1.11
?
x * 47000, SDS-PAGE
Candida glabrata
4.2.1.11
?
x * 47000, SDS-PAGE
Candida parapsilosis
4.2.1.11
?
x * 47000, SDS-PAGE
Clavispora lusitaniae
4.2.1.11
?
x * 47000, SDS-PAGE
Pichia kudriavzevii
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Candida albicans
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Candida glabrata
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Candida parapsilosis
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Clavispora lusitaniae
4.2.1.11
47000
-
x * 47000, SDS-PAGE
Pichia kudriavzevii
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
using anion- and cation exchange chromatography
Candida albicans
4.2.1.11
using anion- and cation exchange chromatography
Candida glabrata
4.2.1.11
using anion- and cation exchange chromatography
Candida parapsilosis
4.2.1.11
using anion- and cation exchange chromatography
Clavispora lusitaniae
4.2.1.11
using anion- and cation exchange chromatography
Pichia kudriavzevii
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2.1.11
60
70
pH 7.4
Candida albicans
4.2.1.11
60
70
pH 7.4
Candida glabrata
4.2.1.11
60
70
pH 7.4
Candida parapsilosis
4.2.1.11
60
70
pH 7.4
Clavispora lusitaniae
4.2.1.11
60
70
pH 7.4
Pichia kudriavzevii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
650658
Pichia kudriavzevii
phosphoenolpyruvate
-
650658
Pichia kudriavzevii
r
4.2.1.11
2-phospho-D-glycerate
-
650658
Candida albicans
phosphoenolpyruvate
-
650658
Candida albicans
r
4.2.1.11
2-phospho-D-glycerate
-
650658
Candida parapsilosis
phosphoenolpyruvate
-
650658
Candida parapsilosis
r
4.2.1.11
2-phospho-D-glycerate
-
650658
Candida glabrata
phosphoenolpyruvate
-
650658
Candida glabrata
r
4.2.1.11
2-phospho-D-glycerate
-
650658
Clavispora lusitaniae
phosphoenolpyruvate
-
650658
Clavispora lusitaniae
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 47000, SDS-PAGE
Candida albicans
4.2.1.11
?
x * 47000, SDS-PAGE
Candida glabrata
4.2.1.11
?
x * 47000, SDS-PAGE
Candida parapsilosis
4.2.1.11
?
x * 47000, SDS-PAGE
Clavispora lusitaniae
4.2.1.11
?
x * 47000, SDS-PAGE
Pichia kudriavzevii