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Literature summary extracted from
- Adenine deaminase activity of the yicP gene product of Escherichia coli (2001), Biosci. Biotechnol. Biochem., 65, 1112-1118.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.4.2 | overexpression in Escherichia coli | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.2 | Mn2+ | required, activation at 1-5 mM, inhibitory above | Escherichia coli |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.2 | 0.8 | - |
adenine | pH 7.5, 37°C | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.2 | Co2+ | 11% of the activity with Mn2+ | Escherichia coli | |
| 3.5.4.2 | Fe2+ | 48% of the activity with Mn2+ | Escherichia coli | |
| 3.5.4.2 | Mn2+ | required, optimal at 1-5 mM, inhibitory above | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.2 | 60000 | - |
2 * 60000, SDS-PAGE | Escherichia coli |
| 3.5.4.2 | 120000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.2 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.2 | recombonant protein | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.2 | 5.97 | - |
pH 7.5, 37°C | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.2 | adenine + H2O | specific substrate | Escherichia coli | hypoxanthine + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.2 | homodimer | 2 * 60000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.2 | 60 | - |
- |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.2 | 6.5 | 7 | - |
Escherichia coli |
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Release 2023.1 (January 2023)
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