Literature summary extracted from
Kim, S.H.; Choi, N.C.
Purification and characterization of subtilisin DJ-4 secreted by Bacillus sp. strain DJ-4 screened from Doen-Jang (2000), Biosci. Biotechnol. Biochem., 64, 1722-1725.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.4.21.62 |
medicine |
fibrinolytic activiy of strain DJ-4, develop the enzyme for its use as a thrombolytic agent |
Bacillus sp. (in: Bacteria) |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.4.21.62 |
Cu2+ |
strain DJ-4, strong inhibition |
Bacillus sp. (in: Bacteria) |
|
3.4.21.62 |
additional information |
strain DJ-4, no inhibition by EDTA or leupeptin |
Bacillus sp. (in: Bacteria) |
|
3.4.21.62 |
phenylmethylsulfonyl fluoride |
strain DJ-4 |
Bacillus sp. (in: Bacteria) |
|
3.4.21.62 |
Zn2+ |
strain DJ-4, strong inhibition |
Bacillus sp. (in: Bacteria) |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
3.4.21.62 |
extracellular |
- |
Bacillus sp. (in: Bacteria) |
- |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.4.21.62 |
additional information |
strain DJ-4, not significantly affected by Ca2+, Co2+, Mg2+, or Mn2+ |
Bacillus sp. (in: Bacteria) |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.4.21.62 |
29000 |
- |
strain DJ-4, SDS-PAGE |
Bacillus sp. (in: Bacteria) |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.4.21.62 |
Bacillus sp. (in: Bacteria) |
- |
isolated from Korean fermented soybean food, Doen-Jang |
- |
3.4.21.62 |
Bacillus sp. (in: Bacteria) DJ-4 |
- |
isolated from Korean fermented soybean food, Doen-Jang |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.4.21.62 |
strain DJ-4 |
Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.4.21.62 |
additional information |
- |
strain DJ-4, specific activity 0.98 |
Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.4.21.62 |
Fibrin + H2O |
strain DJ-4 |
Bacillus sp. (in: Bacteria) |
? |
- |
? |
|
3.4.21.62 |
Fibrin + H2O |
strain DJ-4 |
Bacillus sp. (in: Bacteria) DJ-4 |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.4.21.62 |
monomer |
1 * 29000, strain DJ-4, SDS-PAGE |
Bacillus sp. (in: Bacteria) |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.4.21.62 |
subtilisin DJ-4 |
strain DJ-4 |
Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
3.4.21.62 |
40 |
- |
strain DJ-4 |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Comment |
Organism |
---|
3.4.21.62 |
50 |
- |
strain DJ-4, 80% of the enzyme's activity remains after incubation for 1 h |
Bacillus sp. (in: Bacteria) |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.4.21.62 |
10 |
- |
strain DJ-4 |
Bacillus sp. (in: Bacteria) |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
3.4.21.62 |
4 |
11 |
strain DJ-4 |
Bacillus sp. (in: Bacteria) |