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Literature summary extracted from

  • Kataoka, M.; Nomura, J.I.; Shinohara, M.; Nose, K.; Sakamoto, K.; Shimizu, S.
    Purification and characterization of a novel lactonohydrolase from Agrobacterium tumefaciens (2000), Biosci. Biotechnol. Biochem., 64, 1255-1262.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.25 8-hydroxyquinoline at 2 mM, 50% residual activity Agrobacterium tumefaciens
3.1.1.25 AlCl3 at 2 mM, 66% residual activity Agrobacterium tumefaciens
3.1.1.25 EDTA at 2 mM, 85% residual activity Agrobacterium tumefaciens
3.1.1.25 FeCl2 at 2 mM, 69% residual activity Agrobacterium tumefaciens
3.1.1.25 FeCl3 at 2 mM, 43% residual activity Agrobacterium tumefaciens
3.1.1.25 NaN3 at 2 mM, 84% residual activity Agrobacterium tumefaciens
3.1.1.25 PbCl2 at 2 mM, 62% residual activity Agrobacterium tumefaciens
3.1.1.25 SnCl2 at 2 mM, 62% residual activity Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.25 2.56
-
 3,4-dihydrocoumarin pH 7.4, 30°C Agrobacterium tumefaciens
3.1.1.25 3.59
-
 L-pantoyl lactone pH 7.4, 30°C Agrobacterium tumefaciens
3.1.1.25 4.55
-
 2-coumaranone pH 7.4, 30°C Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.25 26500
-
 2 * 26500, SDS-PAGE Agrobacterium tumefaciens
3.1.1.25 62000
-
 gel filtration Agrobacterium tumefaciens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.25 Agrobacterium tumefaciens
-
-
-
3.1.1.25 Agrobacterium tumefaciens AKU 316
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.25
-
 Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.25 2-coumaranone + H2O 25% of activity compared to L-pantoyl lactone Agrobacterium tumefaciens ?
-
 ?
3.1.1.25 2-coumaranone + H2O 25% of activity compared to L-pantoyl lactone Agrobacterium tumefaciens AKU 316 ?
-
 ?
3.1.1.25 3,4-dihydrocoumarin + H2O 23% of activity compared to L-pantoyl lactone Agrobacterium tumefaciens ?
-
 ?
3.1.1.25 3,4-dihydrocoumarin + H2O 23% of activity compared to L-pantoyl lactone Agrobacterium tumefaciens AKU 316 ?
-
 ?
3.1.1.25 L-pantoyl lactone + H2O stereospecific hydrolysis Agrobacterium tumefaciens L-pantoic acid
-
 ?
3.1.1.25 L-pantoyl lactone + H2O stereospecific hydrolysis Agrobacterium tumefaciens AKU 316 L-pantoic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.25 dimer 2 * 26500, SDS-PAGE Agrobacterium tumefaciens
3.1.1.25 More amino acid sequence analysis Agrobacterium tumefaciens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.25 45
-
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.25 50
-
 stable below Agrobacterium tumefaciens
3.1.1.25 70
-
 20 min, 54% residual activity Agrobacterium tumefaciens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.25 8 8.5
-
 Agrobacterium tumefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.25 4
-
 no activity below Agrobacterium tumefaciens