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Literature summary extracted from

  • Tanaka, T.; Matsuzawa, H.; Ohta, T.
    Substrate specificity of aqualysin I altered by an organic solvent, DMSO (1999), Biosci. Biotechnol. Biochem., 63, 446-448.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.111 expression in Pichia pastoris Thermus aquaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.111 0.04
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
3.4.21.111 0.064
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
3.4.21.111 1
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus
3.4.21.111 1.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
3.4.21.111 2.2
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus
3.4.21.111 9.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.111 Ca2+ binds to low-affinity Ca2+ binding site, binding is essential for thermostabilization Thermus aquaticus
3.4.21.111 La3+ binds to low-affinity Ca2+ binding site as well as to an additional metal ion-binding site that can also be involved in thermostabilization Thermus aquaticus
3.4.21.111 Na+ binds to low-affinity Ca2+ binding site, binding is essential for thermostabilization Thermus aquaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.111 28000
-
 mature protein, SDS-PAGE Thermus aquaticus
3.4.21.111 38000
-
 precursor protein, SDS-PAGE Thermus aquaticus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.21.111 DMSO 10% DMSO alters the substrate specificity of the enzyme, solvent may take part in the substrate recognition of the enzyme Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.111 Thermus aquaticus
-
 YT-1
-
3.4.21.111 Thermus aquaticus YT-1
-
 YT-1
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.111 proteolytic modification intermolecular autocatalytic activation upon heat treatment at 70°C Thermus aquaticus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.111
-
 Thermus aquaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.111 chromogenic succinyl-tripeptide-4-nitroanilide + H2O
-
 Thermus aquaticus ?
-
 ?
3.4.21.111 chromogenic succinyl-tripeptide-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 ?
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ile-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ile-Val-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ile-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ile-Val-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.111 0.1
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
3.4.21.111 0.18
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus
3.4.21.111 1.1
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus
3.4.21.111 1.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
3.4.21.111 5
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.5, 40°C, absence of DMSO Thermus aquaticus
3.4.21.111 8.1
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.5, 40°C, presence of DMSO Thermus aquaticus