BRENDA - Enzyme Database

Enzyme catalysis in microgravity: steady-state kinetic analysis of the isocitrate lyase reaction

Ranaldi, F.; Vanni, P.; Giachetti, E.; Biophys. Chem. 103, 169-177 (2003)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.039
-
isocitrate
pH 7, standard g, cleavage reaction
Pinus pinea
4.1.3.1
0.043
-
isocitrate
pH 7, microgravity, cleavage reaction
Pinus pinea
4.1.3.1
0.053
-
succinate
pH 7, microgravity, condensation reaction
Pinus pinea
4.1.3.1
0.063
-
succinate
pH 7, standard g, condensation reaction
Pinus pinea
4.1.3.1
0.088
-
glyoxylate
pH 7, microgravity, condensation reaction
Pinus pinea
4.1.3.1
0.092
-
glyoxylate
pH 7, standard g, condensation reaction
Pinus pinea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Pinus pinea
-
succinate + glyoxylate
-
Pinus pinea
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Pinus pinea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Pinus pinea
Reaction
EC Number
Reaction
Commentary
Organism
4.1.3.1
isocitrate = succinate + glyoxylate
kinetic mechanism is the same in microgravity and in standard g controls
Pinus pinea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
seed
germinating
Pinus pinea
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
650574
Pinus pinea
succinate + glyoxylate
-
650574
Pinus pinea
r
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.039
-
isocitrate
pH 7, standard g, cleavage reaction
Pinus pinea
4.1.3.1
0.043
-
isocitrate
pH 7, microgravity, cleavage reaction
Pinus pinea
4.1.3.1
0.053
-
succinate
pH 7, microgravity, condensation reaction
Pinus pinea
4.1.3.1
0.063
-
succinate
pH 7, standard g, condensation reaction
Pinus pinea
4.1.3.1
0.088
-
glyoxylate
pH 7, microgravity, condensation reaction
Pinus pinea
4.1.3.1
0.092
-
glyoxylate
pH 7, standard g, condensation reaction
Pinus pinea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Pinus pinea
-
succinate + glyoxylate
-
Pinus pinea
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Pinus pinea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
seed
germinating
Pinus pinea
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
650574
Pinus pinea
succinate + glyoxylate
-
650574
Pinus pinea
r