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Literature summary extracted from

  • Brown, P.; Richardson, C.M.; Mensah, L.M.; O'Hanlon, P.J.; Osborne, N.F.; Pope, A.J.; Walker, G.
    Molecular recognition of tyrosinyl adenylate analogues by prokaryotic tyrosyl tRNA synthetases (1999), Bioorg. Med. Chem., 7, 2473-2485.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.1.1.1 medicine enzyme inhibitors can be used as antibacterial agents Staphylococcus aureus
6.1.1.1 medicine enzyme inhibitors can be used as antibacterial agents Geobacillus stearothermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.1
-
 Staphylococcus aureus
6.1.1.1 X-ray diffraction, at 2.7 A resolution, structure analysis of the enzyme-ligand complex, e.g. with specific synthetic inhibitors, molecular modeling Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.1 L-tyrosinyl 1,4-anhydro-D-ribitol-5-O-phosphate
-
 Geobacillus stearothermophilus
6.1.1.1 L-tyrosinyl 1,4-anhydro-D-ribitol-5-O-phosphate
-
 Staphylococcus aureus
6.1.1.1 L-tyrosinyl 1-beta-naphthyl-1,4-anhydro-D-ribitol-5-O-phosphate
-
 Geobacillus stearothermophilus
6.1.1.1 L-tyrosinyl 1-beta-naphthyl-1,4-anhydro-D-ribitol-5-O-phosphate
-
 Staphylococcus aureus
6.1.1.1 L-tyrosinyl N6-benzoyl adenylate
-
 Geobacillus stearothermophilus
6.1.1.1 L-tyrosinyl uridine-5'-O-phosphate 25% inhibition at 0.1 mM Geobacillus stearothermophilus
6.1.1.1 L-tyrosinyl uridine-5'-O-phosphate
-
 Staphylococcus aureus
6.1.1.1 L-tyrosinyl-2',3'-O-isopropylidene adenylate
-
 Geobacillus stearothermophilus
6.1.1.1 L-tyrosinyl-2',3'-O-isopropylidene adenylate
-
 Staphylococcus aureus
6.1.1.1 L-tyrosinyl-2'-deoxy adenylate
-
 Geobacillus stearothermophilus
6.1.1.1 L-tyrosinyl-2'-deoxy adenylate
-
 Staphylococcus aureus
6.1.1.1 L-tyrosinyl-3'-deoxy adenylate
-
 Geobacillus stearothermophilus
6.1.1.1 L-tyrosinyl-3'-deoxy adenylate
-
 Staphylococcus aureus
6.1.1.1 additional information no inhibition by L-tyrosinyl 1-beta-(E)-(3-ethoxycarbonyl-2-methylprop-2-enyl)-1,4-anhydro-D-ribitol-5-O-phosphate, N-(L-tyrosinyl)-N'-(5'-deoxy-5'-adenosinyl)-sulfamide, and 2(S)-amino-3-(4-hydroxyphenyl)propyl 1-(adenine-9-yl)-(E)-5,6-dideoxy-beta-D-ribohept-5-enfuranuronate hydrochloride Staphylococcus aureus
6.1.1.1 tyrosinyl adenylate
-
 Geobacillus stearothermophilus
6.1.1.1 tyrosinyl adenylate
-
 Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.1 Mg2+ required Staphylococcus aureus
6.1.1.1 Mg2+ required Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.1 ATP + tyrosine + tRNATyr Staphylococcus aureus enzyme plays a key role in protein biosynthesis AMP + Tyr-tRNATyr + diphosphate
-
 ?
6.1.1.1 ATP + tyrosine + tRNATyr Geobacillus stearothermophilus enzyme plays a key role in protein biosynthesis AMP + Tyr-tRNATyr + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.1 Geobacillus stearothermophilus
-
-
-
6.1.1.1 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.1 recombinant enzyme to near homogeneity Staphylococcus aureus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.1 ATP + L-tyrosine + tRNATyr = AMP + diphosphate + L-tyrosyl-tRNATyr the adenine ring is important to the binding of tyrosinyl adenylate to the enzyme, important water-mediated hydrogen interactions Staphylococcus aureus
a
6.1.1.1 ATP + L-tyrosine + tRNATyr = AMP + diphosphate + L-tyrosyl-tRNATyr the adenine ring is important to the binding of tyrosinyl adenylate to the enzyme, important water-mediated hydrogen interactions Geobacillus stearothermophilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.1 ATP + tyrosine + tRNATyr extreme high fidelity in charging the tRNA with an amino acid Staphylococcus aureus AMP + Tyr-tRNATyr + diphosphate
-
 ?
6.1.1.1 ATP + tyrosine + tRNATyr extreme high fidelity in charging the tRNA with an amino acid Geobacillus stearothermophilus AMP + Tyr-tRNATyr + diphosphate
-
 ?
6.1.1.1 ATP + tyrosine + tRNATyr enzyme plays a key role in protein biosynthesis Staphylococcus aureus AMP + Tyr-tRNATyr + diphosphate
-
 ?
6.1.1.1 ATP + tyrosine + tRNATyr enzyme plays a key role in protein biosynthesis Geobacillus stearothermophilus AMP + Tyr-tRNATyr + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.1 tyrosyl tRNA synthetase
-
 Staphylococcus aureus
6.1.1.1 tyrosyl tRNA synthetase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.1 37
-
 assay at Staphylococcus aureus
6.1.1.1 37
-
 assay at Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.1 7.9
-
 assay at Staphylococcus aureus
6.1.1.1 7.9
-
 assay at Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.1 ATP
-
 Staphylococcus aureus
6.1.1.1 ATP
-
 Geobacillus stearothermophilus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.1.1.1 0.0000063
-
 tyrosinyl adenylate pH 7.9, 37°C Geobacillus stearothermophilus
6.1.1.1 0.0000093
-
 L-tyrosinyl-2',3'-O-isopropylidene adenylate pH 7.9, 37°C Geobacillus stearothermophilus
6.1.1.1 0.000011
-
 L-tyrosinyl adenylate pH 7.9, 37°C Staphylococcus aureus
6.1.1.1 0.000026
-
 L-tyrosinyl-2',3'-O-isopropylidene adenylate pH 7.9, 37°C Staphylococcus aureus
6.1.1.1 0.00021
-
 L-tyrosinyl uridine-5'-O-phosphate pH 7.9, 37°C Staphylococcus aureus
6.1.1.1 0.00068
-
 L-tyrosinyl N6-benzoyl adenylate pH 7.9, 37°C Geobacillus stearothermophilus
6.1.1.1 0.0057
-
 L-tyrosinyl 1-beta-naphthyl-1,4-anhydro-D-ribitol-5-O-phosphate pH 7.9, 37°C Geobacillus stearothermophilus
6.1.1.1 0.018
-
 L-tyrosinyl-2'-deoxy adenylate pH 7.9, 37°C Geobacillus stearothermophilus
6.1.1.1 0.036
-
 L-tyrosinyl-3'-deoxy adenylate pH 7.9, 37°C Geobacillus stearothermophilus
6.1.1.1 0.076
-
 L-tyrosinyl 1,4-anhydro-D-ribitol-5-O-phosphate pH 7.9, 37°C Geobacillus stearothermophilus
6.1.1.1 0.1
-
 L-tyrosinyl-2'-deoxy adenylate pH 7.9, 37°C Staphylococcus aureus
6.1.1.1 0.14
-
 L-tyrosinyl-3'-deoxy adenylate pH 7.9, 37°C Staphylococcus aureus
6.1.1.1 0.15
-
 L-tyrosinyl 1-beta-naphthyl-1,4-anhydro-D-ribitol-5-O-phosphate pH 7.9, 37°C Staphylococcus aureus
6.1.1.1 0.42
-
 L-tyrosinyl 1,4-anhydro-D-ribitol-5-O-phosphate pH 7.9, 37°C Staphylococcus aureus