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Literature summary extracted from

  • DeSantis, G.; Liu, J.; Clark, D.P.; Heine, A.; Wilson, I.A.; Wong, C.H.
    Structure-based mutagenesis approaches toward expanding the substrate specificity of D-2-deoxyribose-5-phosphate aldolase (2003), Bioorg. Med. Chem., 11, 43-52.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.4
-
 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.4 G205E decreased activity towards the natural substrate Escherichia coli
4.1.2.4 K172E decreased activity towards the natural substrate Escherichia coli
4.1.2.4 R207E altered substrate specificity Escherichia coli
4.1.2.4 S238D altered substrate specificity Escherichia coli
4.1.2.4 S239E decreased activity towards the natural substrate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.4 0.64
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, wild-type Escherichia coli
4.1.2.4 4.3
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S239E mutant Escherichia coli
4.1.2.4 33
-
 D-2-deoxyribose pH 7.5, 25°C, R207E mutant Escherichia coli
4.1.2.4 39
-
 D-2-deoxyribose pH 7.5, 25°C, S238D mutant Escherichia coli
4.1.2.4 57
-
 D-2-deoxyribose pH 7.5, 25°C, wild-type Escherichia coli
4.1.2.4 61
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S238D mutant Escherichia coli
4.1.2.4 63
-
 D-2-deoxyribose pH 7.5, 25°C, K172E mutant Escherichia coli
4.1.2.4 67
-
 D-2-deoxyribose pH 7.5, 25°C, S239E mutant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.4 2-deoxy-D-ribose 5-phosphate Escherichia coli
-
 D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.4 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.4
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.4 2-deoxy-D-ribose 5-phosphate
-
 Escherichia coli D-glyceraldehyde 3-phosphate + acetaldehyde
-
 r
4.1.2.4 3-azidopropanal + 2 acetaldehyde
-
 Escherichia coli (4R,6R)-6-(2-azidoethyl)-4-hydroxyoxan-2-one + 2 H+
-
 ?
4.1.2.4 D-2-deoxyribose good substrate for the S238D mutant, weak substrate for the wild-type enzyme Escherichia coli glyceraldehyde + acetaldehyde
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.4 0.022
-
 D-2-deoxyribose pH 7.5, 25°C, K172E mutant Escherichia coli
4.1.2.4 0.064
-
 D-2-deoxyribose pH 7.5, 25°C, R207E mutant Escherichia coli
4.1.2.4 0.107
-
 D-2-deoxyribose pH 7.5, 25°C, wild-type Escherichia coli
4.1.2.4 0.175
-
 D-2-deoxyribose pH 7.5, 25°C, S239E mutant Escherichia coli
4.1.2.4 0.21
-
 D-2-deoxyribose pH 7.5, 25°C, S238D mutant Escherichia coli
4.1.2.4 0.58
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S238D mutant Escherichia coli
4.1.2.4 6.08
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S238D mutant Escherichia coli
4.1.2.4 41
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, S239E mutant Escherichia coli
4.1.2.4 68
-
 2-deoxy-D-ribose 5-phosphate pH 7.5, 25°C, wild-type Escherichia coli