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Literature summary extracted from

  • Zhang, S.; Wilson, D.B.; Ganem, B.
    An engineered chorismate mutase with allosteric regulation (2003), Bioorg. Med. Chem., 11, 3109-3114.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 expression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
5.4.99.5 DELTA102-285 hybrid of chorismate mutase and allosteric domain from P-protein without prephrenate dehydratase Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 0.226
-
 chorismate 37°C, pH 7.8, in presence of P-protein Escherichia coli
5.4.99.5 0.296
-
 chorismate 37°C, pH 7.8 Escherichia coli
5.4.99.5 0.549
-
 prephenate 37°C, pH 7.8, in presence of P-protein Escherichia coli
5.4.99.5 0.59 1 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine Escherichia coli
5.4.99.5 0.628
-
 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine Escherichia coli
5.4.99.5 1.036
-
 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine Escherichia coli
5.4.99.5 2.547
-
 chorismate 37°C, pH 7.8, DELTA102-285 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.5 Chorismate Escherichia coli phenylalanine biosynthesis Prephenate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate reversible only in presence of P-protein Escherichia coli Prephenate
-
 r
5.4.99.5 Chorismate phenylalanine biosynthesis Escherichia coli Prephenate
-
 r

Subunits

EC Number Subunits Comment Organism
5.4.99.5 dimer SDS-PAGE and gel filtration Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 chorismate mutase
-
 Escherichia coli
5.4.99.5 EcCM-R
-
 Escherichia coli
5.4.99.5 P-protein
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 94100
-
 prephenate 37°C, pH 7.8, in presence of P-protein Escherichia coli
5.4.99.5 94140
-
 prephenate 37°C, pH 7.8, in presence of P-protein Escherichia coli
5.4.99.5 140500
-
 chorismate 37°C, pH 7.8, in presence of P-protein Escherichia coli
5.4.99.5 141000
-
 chorismate 37°C, pH 7.8, in presence of P-protein Escherichia coli
5.4.99.5 148900
-
 chorismate 37°C, pH 7.8, chorismate mutase Escherichia coli
5.4.99.5 149000
-
 chorismate 37°C, pH 7.8, chorismate mutase Escherichia coli
5.4.99.5 234000
-
 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine Escherichia coli
5.4.99.5 234100
-
 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.05 mM phenylalanine Escherichia coli
5.4.99.5 253000
-
 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine Escherichia coli
5.4.99.5 253400
-
 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 0.5 mM phenylalanine Escherichia coli
5.4.99.5 257900
-
 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine Escherichia coli
5.4.99.5 258000
-
 chorismate 37°C, pH 7.8, DELTA102-285 in presence of 2 mM phenylalanine Escherichia coli
5.4.99.5 365600
-
 chorismate 37°C, pH 7.8, DELTA102-285 Escherichia coli
5.4.99.5 366000
-
 chorismate 37°C, pH 7.8, DELTA102-285 Escherichia coli