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Literature summary extracted from

  • Yasuda, K.; Okumura, T.; Okada, H.; Nakajima, T.; Aoki, J.; Arai, H.; Inoue, K.; Nishizawa, M.; Ito, S.; Kanzaki, H.
    Platelet-activating factor acetylhydrolase isoforms I and II in human uterus. Comparisons with pregnant uterus and myoma (2001), Biol. Reprod., 64, 339-344.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.47 cytosol at least 2 isozymes I and II Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.47 29000
-
 1 * 29000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, fetal brain enzyme, SDS-PAGE, 1 * 20000-25000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, kidney and uterus enzyme, SDS-PAGE Homo sapiens
3.1.1.47 30000
-
 1 * 29000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, fetal brain enzyme, SDS-PAGE, 1 * 20000-25000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, kidney and uterus enzyme, SDS-PAGE Homo sapiens
3.1.1.47 45000
-
 1 * 29000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, fetal brain enzyme, SDS-PAGE, 1 * 20000-25000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, kidney and uterus enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O Homo sapiens i.e. platelet-activation factor 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.47 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.47 brain fetal Homo sapiens
-
3.1.1.47 kidney
-
 Homo sapiens
-
3.1.1.47 myoma cell at least 2 isozymes I and II, increased amount of isozyme I beta-subunit Homo sapiens
-
3.1.1.47 myometrium at least 2 isozymes I and II Homo sapiens
-
3.1.1.47 uterus isozyme I activity is lower in pregnant uterus compared to the nonpregnant one Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.47 0.000008
-
 cytosolic fraction, nonpregnant uterus Homo sapiens
3.1.1.47 0.000073
-
 cytosolic fraction, pregnant uterine myometrium Homo sapiens
3.1.1.47 0.000221
-
 cytosolic fraction, myoma Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.47 2-acetyl-1-hexadecyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?
3.1.1.47 2-hexadecyl-1-alkyl-sn-glycero-3-phosphocholine + H2O i.e. platelet-activation factor Homo sapiens 1-hexadecyl-sn-glycero-3-phosphocholine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.47 More isozyme I is a trimer of 2 alpha-subunits and one beta-subunit, isozyme II is a monomer Homo sapiens
3.1.1.47 trimer 1 * 29000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, fetal brain enzyme, SDS-PAGE, 1 * 20000-25000, alpha1-subunit, + 1 * 30000, alpha2-subunit, + 1 * 45000, beta-subunit, kidney and uterus enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.1.1.47 PAF acetylhydrolase
-
 Homo sapiens
3.1.1.47 PAF-AH
-
 Homo sapiens
3.1.1.47 platelet-activating factor acetylhydrolase
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.47 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.47 7.4
-
 assay at Homo sapiens