Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Obama, T.; Fujii, S.; Ikezawa, H.; Ikeda, K.; Imagawa, M.; Tsukamoto, K.
    His151 and His296 are the acid-base catalytic residues of Bacillus cereus sphingomyelinase in sphingomyelin hydrolysis (2003), Biol. Pharm. Bull., 26, 920-926.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 expression of nSMase in Bacillus brevis 47 Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 H151A 0.6% of wild-type nSMase activity Bacillus cereus
3.1.4.12 H151D no nSMase activity Bacillus cereus
3.1.4.12 H151E no nSMase activity Bacillus cereus
3.1.4.12 H151F no nSMase activity Bacillus cereus
3.1.4.12 H151K no nSMase activity Bacillus cereus
3.1.4.12 H151N no nSMase activity Bacillus cereus
3.1.4.12 H151Q 8.3% of wild-type nSMase activity Bacillus cereus
3.1.4.12 H151R no nSMase activity Bacillus cereus
3.1.4.12 H151Y no nSMase activity Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.35
-
 sphingomyelin 37°C, pH 7.5 Bacillus cereus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.12 extracellular extracellular soluble toxin that induces hemolysis by acting on sphingomyelin-rich erythrocytes of mammals such as ruminants Bacillus cereus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Co2+ can replace Mg2+ Bacillus cereus
3.1.4.12 Mg2+ required for activity, Glu53 act as a ligand for the essential Mg2+ Bacillus cereus
3.1.4.12 Mn2+ can replace Mg2+ Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Bacillus cereus
-
 N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Bacillus cereus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 recombinant nSMase Bacillus cereus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 2-hexadecanoylamino-4-nitrophenylphosphocholine + H2O
-
 Bacillus cereus 2-hexadecanoylamino-4-nitrophenol + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Bacillus cereus N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 neutral sphingomyelinase
-
 Bacillus cereus
3.1.4.12 nSMase
-
 Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.12 3.6
-
 sphingomyelin 37°C, pH 7.5 Bacillus cereus