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Literature summary extracted from

  • Michihara, A.; Akasaki, K.; Yamori, Y.; Tsuji, H.
    Purification and characterization of mouse mevalonate pyrophosphate decarboxylase (2002), Biol. Pharm. Bull., 25, 302-306.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.33 ATP required Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.33 DTNB 10 mM, 60% inhibition Mus musculus
4.1.1.33 iodoacetic acid 10 mM, 15% inhibition Mus musculus
4.1.1.33 NEM 10 mM, 40% inhibition Mus musculus
4.1.1.33 p-chloromercuribenzene sulfonic acid 10 mM, 50% inhibition Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.33 0.01
-
 mevalonate diphosphate pH 7.2 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.33 cytosol
-
 Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.33 Co2+ divalent metal ion required, Mg2+ can be substituted by Mn2+ or Co2+ Mus musculus
4.1.1.33 Mg2+ divalent metal ion required, Mg2+ can be substituted by Mn2+ or Co2+ Mus musculus
4.1.1.33 Mn2+ divalent metal ion required, Mg2+ can be substituted by Mn2+ or Co2+ Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.33 46000
-
 2 * 46000, SDS-PAGE Mus musculus
4.1.1.33 100000
-
 gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.33 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.33
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.33 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.33 0.2327
-
-
 Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.33 ATP + 5-diphosphomevalonate
-
 Mus musculus ADP + phosphate + isopentenyl diphosphate + CO2
-
 ?
4.1.1.33 CTP + 5-diphosphomevalonate 12% of the activity with ATP Mus musculus CDP + phosphate + isopentenyl diphosphate + CO2
-
 ?
4.1.1.33 GTP + 5-diphosphomevalonate 25% of the activity with ATP Mus musculus GDP + phosphate + isopentenyl diphosphate + CO2
-
 ?
4.1.1.33 UTP + 5-diphosphomevalonate 13% of the activity with ATP Mus musculus UDP + phosphate + isopentenyl diphosphate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.33 dimer 2 * 46000, SDS-PAGE Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.33 4 7
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.33 4 10 pH 3.0: about 5% of maximal activity, pH 4.0-7.0: maximal activity, pH 10.0: about 40% of maximal activity Mus musculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.33 Mus musculus isoelectric focusing
-
 5