Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chen, J.M.; Fortunato, M.; Stevens, R.A.E.; Barrett, A.J.
    Activation of progelatinase A by mammalian lugumain, a recently discovered cysteine proteinase (2001), Biol. Chem., 382, 777-783.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.34 stable overexpression in HEK 293 cells and in C13 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.34 cystatin
-
 Sus scrofa
3.4.22.34 additional information no inhibition by PMSF, EDTA and 1,10-phenanthroline Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.34 lysosome
-
 Homo sapiens 5764
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.34 progelatinase A + H2O Homo sapiens in cell culture of human fibrosarcoma HT1080 cells, 72 kDa proform, activation by cleavage of an asparaginyl peptide bond at Asn109-Tyr110 or Asn111-Phe112 to form the N-terminus of the mature active gelatinase gelatinase A + ? 62 kDa mature form ?
3.4.22.34 protein + H2O Sus scrofa
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.34 Homo sapiens
-
-
-
3.4.22.34 Sus scrofa
-
 purified enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.22.34 proteolytic modification the 56 kDa proform is cleavaed to the active 46 kDa and 36 kDa forms Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.34 recombinant enzyme Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.34 kidney
-
 Sus scrofa
-
3.4.22.34 additional information no activity in native HEK 293 cell Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.34 gelatinase + H2O 62 kDa active form, cleavage site is Asn430 Homo sapiens gelatinase fragment 36 kDa active form ?
3.4.22.34 gelatinase + H2O 62 kDa active form, cleavage site is Asn430 Sus scrofa gelatinase fragment 36 kDa active form ?
3.4.22.34 additional information no activity with pro-/gelatinase B Homo sapiens ?
-
 ?
3.4.22.34 additional information no activity with pro-/gelatinase B Sus scrofa ?
-
 ?
3.4.22.34 progelatinase A + H2O 72 kDa human proform, activation by cleavage of an asparaginyl peptide bonde to form the N-terminus of the mature active gelatinase Sus scrofa gelatinase + ? 62 kDa mature form ?
3.4.22.34 progelatinase A + H2O 72 kDa proform, activation by cleavage of an asparaginyl peptide bonde to form the N-terminus of the mature active gelatinase Homo sapiens gelatinase + ? 62 kDa mature form ?
3.4.22.34 progelatinase A + H2O in cell culture of human fibrosarcoma HT1080 cells, 72 kDa proform, activation by cleavage of an asparaginyl peptide bond at Asn109-Tyr110 or Asn111-Phe112 to form the N-terminus of the mature active gelatinase Homo sapiens gelatinase A + ? 62 kDa mature form ?
3.4.22.34 protein + H2O
-
 Sus scrofa peptides
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.34 lysosomal cysteine proteinase
-
 Homo sapiens
3.4.22.34 lysosomal cysteine proteinase
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.34 30
-
 assay at Homo sapiens
3.4.22.34 30
-
 assay at Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.34 5.8
-
 assay at Homo sapiens
3.4.22.34 5.8
-
 aasy at Sus scrofa