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Literature summary extracted from

  • Banbula, A.; Mak, P.; Smoluch, M.; Travis, J.; Potempa, J.
    Arginine-specific cysteine proteinase from Porphyromonas gingivalis as a convenient tool in protein chemistry (2001), Biol. Chem., 382, 1399-1404.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.37 dithiothreitol activates Porphyromonas gingivalis
3.4.22.37 L-cysteine activates Porphyromonas gingivalis
3.4.22.37 additional information enzyme activity and cleavage pattern are not affected by 0.1% SDS, 1% Triton X-100, or 1% octyl- and decylpyranoside Porphyromonas gingivalis
3.4.22.37 Urea 3fold activation at 6 M, probably due to unfolding of the substrate azocasein, which enhances the enzymes sensitivity for proteolytic cleavage Porphyromonas gingivalis

Application

EC Number Application Comment Organism
3.4.22.37 molecular biology enzyme is a convenient tool for protein chemistry due to its stability and activity under conditions of high detergent concentration used in protein solubilization and purification Porphyromonas gingivalis

General Stability

EC Number General Stability Organism
3.4.22.37 1% SDS slightly decreases enzyme activity Porphyromonas gingivalis
3.4.22.37 enzyme is completely inactivated in 8 M urea Porphyromonas gingivalis
3.4.22.37 RgpB is stable and active in buffers containing 6 M urea, 0.1% SDS, 1% Triton X-100, and 1% octyl or decylpyranoside Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.37 additional information enzyme activity and cleavage pattern are not affected by 0.1% SDS, 1% Triton X-100, 1% octyl- and decylpyranoside Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.37 protein + H2O Porphyromonas gingivalis
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.37 Porphyromonas gingivalis
-
 enzyme form RgpB
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.37 alpha-globin + H2O absolutely specific cleavage of all Arg-Xaa peptides bonds, in presence of 4 M urea, because alpha-globin is not soluble at neutral pH Porphyromonas gingivalis ?
-
 ?
3.4.22.37 azocasein + H2O
-
 Porphyromonas gingivalis ?
-
 ?
3.4.22.37 beta-globin + H2O absolutely specific cleavage of all Arg-Xaa peptides bonds, in presence of 4 M urea, because beta-globin is not soluble at neutral pH Porphyromonas gingivalis ?
-
 ?
3.4.22.37 Lysozyme + H2O absolutely specific cleavage of all Arg-Xaa peptides bonds Porphyromonas gingivalis ?
-
 ?
3.4.22.37 protein + H2O
-
 Porphyromonas gingivalis peptides
-
 ?
3.4.22.37 ribonuclease A + H2O absolutely specific cleavage of all Arg-Xaa peptides bonds Porphyromonas gingivalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.37 Arginine-specific cysteine protease
-
 Porphyromonas gingivalis
3.4.22.37 RgpB
-
 Porphyromonas gingivalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.37 37
-
 assay at Porphyromonas gingivalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.37 7.6
-
 assay at Porphyromonas gingivalis