BRENDA - Enzyme Database

Human and rat dipeptidyl peptidase III: biochemical and mass spectrometric arguments for similarities and differences

Abramic, M.; Schleuder, D.; Dolovcak, L.; Schroder, W.; Strupat, K.; Sagi, D.; Peter-Katalini, J.; Vitale, L.; Biol. Chem. 381, 1233-1243 (2000) View publication on PubMed

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.14.4
amastatin
30% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
angiotensin II
-
Homo sapiens
3.4.14.4
angiotensin II
-
Rattus norvegicus
3.4.14.4
angiotensin III
-
Homo sapiens
3.4.14.4
angiotensin III
-
Rattus norvegicus
3.4.14.4
bestatin
10% inhibition at 0.1 mM
Homo sapiens
3.4.14.4
bestatin
35% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
Co2+
activation up to 50% at 0.001-0.0001 mM, inbhibitory above 0.002 mM
Rattus norvegicus
3.4.14.4
E-64
i.e. L-trans-epoxysuccinyl-leucylamido(4-guanidino)butane, 10% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
EDTA
67% inhibition at 5 mM
Homo sapiens
3.4.14.4
Gly-Gly-Phe-Leu
fragment of Leu-enkephalin
Homo sapiens
3.4.14.4
Gly-Gly-Phe-Leu
fragment of Leu-enkephalin
Rattus norvegicus
3.4.14.4
His-Phe-Arg-Trp
fragment of alpha-melanocyte-stimulating hormone
Homo sapiens
3.4.14.4
His-Phe-Arg-Trp
fragment of alpha-melanocyte-stimulating hormone
Rattus norvegicus
3.4.14.4
Leu-Trp-Met-Arg-Phe
-
Homo sapiens
3.4.14.4
Leu-Trp-Met-Arg-Phe
-
Rattus norvegicus
3.4.14.4
leupeptin
10% inhibition at 0.1 mM
Homo sapiens
3.4.14.4
Lys-Val-Ile-Leu-Phe
fragment of hydra head activator
Homo sapiens
3.4.14.4
Lys-Val-Ile-Leu-Phe
fragment of hydra ‘head activator’
Rattus norvegicus
3.4.14.4
Nalpha-tosyl-L-Phe-chloromethyl ketone
60% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
o-phenanthroline
93% inhibition at 1 mM
Homo sapiens
3.4.14.4
o-phenanthroline
50% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
PMSF
40% inhibition at 1 mM
Rattus norvegicus
3.4.14.4
probestin
85% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
Proctolin
-
Homo sapiens
3.4.14.4
Proctolin
-
Rattus norvegicus
3.4.14.4
puromycin
15% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
Tyr-Gly-Gly-Phe-Leu
Leu-enkephalin
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe-Leu
Leu-enkephalin
Rattus norvegicus
3.4.14.4
Tyr-Gly-Gly-Phe-Met
Met-enkephalin
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe-Met
Met-enkephalin
Rattus norvegicus
3.4.14.4
Zn2+
50% inhibition at 0.032 mM
Homo sapiens
3.4.14.4
Zn2+
50% inhibition at 0.001 mM
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.14.4
0.00621
-
Arg-Arg-2-naphthylamide
pH 7.4, 37°C
Homo sapiens
3.4.14.4
0.00651
-
Arg-Arg-2-naphthylamide
pH 7.4, 37°C
Rattus norvegicus
3.4.14.4
0.00788
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C
Rattus norvegicus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.14.4
Co2+
activation up to 2fold at micromolar concentrations
Homo sapiens
3.4.14.4
Co2+
activation up to 50% at 0.001-0.0001 mM, inbhibitory above 0.002 mM
Rattus norvegicus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.14.4
82500
-
x * 82500, mass spectrometry
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.14.4
Homo sapiens
-
-
-
3.4.14.4
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.14.4
-
Homo sapiens
3.4.14.4
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.14.4
erythrocyte
-
Homo sapiens
-
3.4.14.4
erythrocyte
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.14.4
Ala-Ala-2-naphthylamide + H2O
poor substrate
650445
Homo sapiens
Ala-Ala + 2-naphthylamine
-
650445
Homo sapiens
?
3.4.14.4
Ala-Ala-2-naphthylamide + H2O
poor substrate
650445
Rattus norvegicus
Ala-Ala + 2-naphthylamine
-
650445
Rattus norvegicus
?
3.4.14.4
Arg-Arg-2-naphthylamide + H2O
-
650445
Homo sapiens
Arg-Arg + 2-naphthylamine
-
650445
Homo sapiens
?
3.4.14.4
Arg-Arg-2-naphthylamide + H2O
-
650445
Rattus norvegicus
Arg-Arg + 2-naphthylamine
-
650445
Rattus norvegicus
?
3.4.14.4
Lys-Ala-4-methoxy-2-naphthylamide + H2O
poor substrate
650445
Homo sapiens
Lys-Ala + 4-methoxy-2-naphthylamine
-
650445
Homo sapiens
?
3.4.14.4
Lys-Ala-4-methoxy-2-naphthylamide + H2O
poor substrate
650445
Rattus norvegicus
Lys-Ala + 4-methoxy-2-naphthylamine
-
650445
Rattus norvegicus
?
3.4.14.4
additional information
enzyme prefers a hydrophobic residue at P1’ position
650445
Homo sapiens
?
-
650445
Homo sapiens
?
3.4.14.4
additional information
enzyme prefers a hydrophobic residue at P1’ position
650445
Rattus norvegicus
?
-
650445
Rattus norvegicus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.14.4
?
x * 82500, mass spectrometry
Homo sapiens
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.14.4
7.4
-
45% of maximal activity
Homo sapiens
3.4.14.4
7.4
-
45% of maximal activity
Rattus norvegicus
3.4.14.4
8
-
-
Rattus norvegicus
3.4.14.4
8
-
80% of maximal activity
Homo sapiens
3.4.14.4
8.7
8.8
-
Homo sapiens
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.4.14.4
0.000024
-
angiotensin III
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00009
-
angiotensin III
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.00009
-
Leu-Trp-Met-Arg-Phe
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.00013
-
Leu-Trp-Met-Arg-Phe
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00015
-
Proctolin
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.00021
-
Lys-Val-Ile-Leu-Phe
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.0004
-
Lys-Val-Ile-Leu-Phe
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00043
-
Proctolin
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00066
-
angiotensin II
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00083
-
angiotensin II
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.0065
-
His-Phe-Arg-Trp
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.01
-
His-Phe-Arg-Trp
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.027
-
Tyr-Gly-Gly-Phe-Leu
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.027
-
Tyr-Gly-Gly-Phe-Met
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.0337
-
Tyr-Gly-Gly-Phe-Leu
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.0343
-
Tyr-Gly-Gly-Phe-Met
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.05
-
Gly-Gly-Phe-Leu
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.074
-
Gly-Gly-Phe-Leu
37°C, pH 7.4
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.14.4
amastatin
30% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
angiotensin II
-
Homo sapiens
3.4.14.4
angiotensin II
-
Rattus norvegicus
3.4.14.4
angiotensin III
-
Homo sapiens
3.4.14.4
angiotensin III
-
Rattus norvegicus
3.4.14.4
bestatin
35% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
bestatin
10% inhibition at 0.1 mM
Homo sapiens
3.4.14.4
Co2+
activation up to 50% at 0.001-0.0001 mM, inbhibitory above 0.002 mM
Rattus norvegicus
3.4.14.4
E-64
i.e. L-trans-epoxysuccinyl-leucylamido(4-guanidino)butane, 10% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
EDTA
67% inhibition at 5 mM
Homo sapiens
3.4.14.4
Gly-Gly-Phe-Leu
fragment of Leu-enkephalin
Homo sapiens
3.4.14.4
Gly-Gly-Phe-Leu
fragment of Leu-enkephalin
Rattus norvegicus
3.4.14.4
His-Phe-Arg-Trp
fragment of alpha-melanocyte-stimulating hormone
Homo sapiens
3.4.14.4
His-Phe-Arg-Trp
fragment of alpha-melanocyte-stimulating hormone
Rattus norvegicus
3.4.14.4
Leu-Trp-Met-Arg-Phe
-
Homo sapiens
3.4.14.4
Leu-Trp-Met-Arg-Phe
-
Rattus norvegicus
3.4.14.4
leupeptin
10% inhibition at 0.1 mM
Homo sapiens
3.4.14.4
Lys-Val-Ile-Leu-Phe
fragment of hydra head activator
Homo sapiens
3.4.14.4
Lys-Val-Ile-Leu-Phe
fragment of hydra ‘head activator’
Rattus norvegicus
3.4.14.4
Nalpha-tosyl-L-Phe-chloromethyl ketone
60% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
o-phenanthroline
93% inhibition at 1 mM
Homo sapiens
3.4.14.4
o-phenanthroline
50% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
PMSF
40% inhibition at 1 mM
Rattus norvegicus
3.4.14.4
probestin
85% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
Proctolin
-
Homo sapiens
3.4.14.4
Proctolin
-
Rattus norvegicus
3.4.14.4
puromycin
15% inhibition at 0.1 mM
Rattus norvegicus
3.4.14.4
Tyr-Gly-Gly-Phe-Leu
Leu-enkephalin
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe-Leu
Leu-enkephalin
Rattus norvegicus
3.4.14.4
Tyr-Gly-Gly-Phe-Met
Met-enkephalin
Homo sapiens
3.4.14.4
Tyr-Gly-Gly-Phe-Met
Met-enkephalin
Rattus norvegicus
3.4.14.4
Zn2+
50% inhibition at 0.001 mM
Rattus norvegicus
3.4.14.4
Zn2+
50% inhibition at 0.032 mM
Homo sapiens
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.4.14.4
0.000024
-
angiotensin III
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00009
-
angiotensin III
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.00009
-
Leu-Trp-Met-Arg-Phe
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.00013
-
Leu-Trp-Met-Arg-Phe
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00015
-
Proctolin
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.00021
-
Lys-Val-Ile-Leu-Phe
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.0004
-
Lys-Val-Ile-Leu-Phe
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00043
-
Proctolin
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00066
-
angiotensin II
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.00083
-
angiotensin II
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.0065
-
His-Phe-Arg-Trp
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.01
-
His-Phe-Arg-Trp
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.027
-
Tyr-Gly-Gly-Phe-Leu
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.027
-
Tyr-Gly-Gly-Phe-Met
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.0337
-
Tyr-Gly-Gly-Phe-Leu
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.0343
-
Tyr-Gly-Gly-Phe-Met
37°C, pH 7.4
Rattus norvegicus
3.4.14.4
0.05
-
Gly-Gly-Phe-Leu
37°C, pH 7.4
Homo sapiens
3.4.14.4
0.074
-
Gly-Gly-Phe-Leu
37°C, pH 7.4
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.14.4
0.00621
-
Arg-Arg-2-naphthylamide
pH 7.4, 37°C
Homo sapiens
3.4.14.4
0.00651
-
Arg-Arg-2-naphthylamide
pH 7.4, 37°C
Rattus norvegicus
3.4.14.4
0.00788
-
Arg-Arg-2-naphthylamide
pH 8.0, 37°C
Rattus norvegicus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.14.4
Co2+
activation up to 2fold at micromolar concentrations
Homo sapiens
3.4.14.4
Co2+
activation up to 50% at 0.001-0.0001 mM, inbhibitory above 0.002 mM
Rattus norvegicus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.14.4
82500
-
x * 82500, mass spectrometry
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.14.4
-
Homo sapiens
3.4.14.4
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.14.4
erythrocyte
-
Homo sapiens
-
3.4.14.4
erythrocyte
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.14.4
Ala-Ala-2-naphthylamide + H2O
poor substrate
650445
Homo sapiens
Ala-Ala + 2-naphthylamine
-
650445
Homo sapiens
?
3.4.14.4
Ala-Ala-2-naphthylamide + H2O
poor substrate
650445
Rattus norvegicus
Ala-Ala + 2-naphthylamine
-
650445
Rattus norvegicus
?
3.4.14.4
Arg-Arg-2-naphthylamide + H2O
-
650445
Homo sapiens
Arg-Arg + 2-naphthylamine
-
650445
Homo sapiens
?
3.4.14.4
Arg-Arg-2-naphthylamide + H2O
-
650445
Rattus norvegicus
Arg-Arg + 2-naphthylamine
-
650445
Rattus norvegicus
?
3.4.14.4
Lys-Ala-4-methoxy-2-naphthylamide + H2O
poor substrate
650445
Homo sapiens
Lys-Ala + 4-methoxy-2-naphthylamine
-
650445
Homo sapiens
?
3.4.14.4
Lys-Ala-4-methoxy-2-naphthylamide + H2O
poor substrate
650445
Rattus norvegicus
Lys-Ala + 4-methoxy-2-naphthylamine
-
650445
Rattus norvegicus
?
3.4.14.4
additional information
enzyme prefers a hydrophobic residue at P1’ position
650445
Homo sapiens
?
-
650445
Homo sapiens
?
3.4.14.4
additional information
enzyme prefers a hydrophobic residue at P1’ position
650445
Rattus norvegicus
?
-
650445
Rattus norvegicus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.14.4
?
x * 82500, mass spectrometry
Homo sapiens
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.14.4
7.4
-
45% of maximal activity
Homo sapiens
3.4.14.4
7.4
-
45% of maximal activity
Rattus norvegicus
3.4.14.4
8
-
-
Rattus norvegicus
3.4.14.4
8
-
80% of maximal activity
Homo sapiens
3.4.14.4
8.7
8.8
-
Homo sapiens