Any feedback?
Literature summary extracted from
- Identification and characterization of spodoptera frugiperda furin: a thermostable subtilisin-like endopeptidase (1998), Biol. Chem., 379, 1433-1440.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.21.75 | cDNA sequenced, cloned from a cDNA library of Sf9 cells, overexpressed from a baculovirus vector in Sf9 cells | Spodoptera frugiperda |
| 3.4.21.75 | recombinant Bfurin expressed in Sf9 cells | Bos taurus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.75 | chymostatin | - |
Bos taurus |  |
| 3.4.21.75 | chymostatin | - |
Spodoptera frugiperda | |
| 3.4.21.75 | CuSO4 | - |
Bos taurus | |
| 3.4.21.75 | CuSO4 | - |
Spodoptera frugiperda | |
| 3.4.21.75 | EDTA | - |
Bos taurus | |
| 3.4.21.75 | EDTA | - |
Spodoptera frugiperda | |
| 3.4.21.75 | HgCl2 | - |
Bos taurus | |
| 3.4.21.75 | HgCl2 | - |
Spodoptera frugiperda | |
| 3.4.21.75 | iodoacetamide | about 50% inhibition | Bos taurus | |
| 3.4.21.75 | iodoacetamide | about 50% inhibition | Spodoptera frugiperda | |
| 3.4.21.75 | additional information | largely unaffected by aprotinin, leupeptin, pepstatin A, [4-amidino-phenyl]-methansulfonylfluoride, (4-[2-aminoethyl]-benzolsulfonylfluoride), phosphoramidon, bestatin, phenylmethylsulfonylfluoride, and antipain | Bos taurus | |
| 3.4.21.75 | additional information | largely unaffected by aprotinin, leupeptin, pepstatin A, [4-amidino-phenyl]-methansulfonylfluoride, (4-[2-aminoethyl]-benzolsulfonylfluoride), phosphoramidon, bestatin, phenylmethylsulfonylfluoride, and antipain | Spodoptera frugiperda | |
| 3.4.21.75 | ZnCl2 | - |
Bos taurus | |
| 3.4.21.75 | ZnCl2 | - |
Spodoptera frugiperda | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.21.75 | Golgi apparatus | trans Golgi network | Spodoptera frugiperda | 5794 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.21.75 | Ca2+ | activated at concentrations below 0.01 mM | Spodoptera frugiperda | |
| 3.4.21.75 | Ca2+ | activated between 0.1 and 10 mM, optimum at about 1 mM | Bos taurus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.75 | additional information | Spodoptera frugiperda | plays an important role in posttranslatioal protein processing | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.21.75 | Bos taurus | - |
- |
- |
| 3.4.21.75 | Spodoptera frugiperda | Q26489 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.21.75 | FPV precursor molecule HA0 + H2O | - |
Bos taurus | subunit HA1 + subunit HA2 | - |
? | |
| 3.4.21.75 | FPV precursor molecule HA0 + H2O | - |
Spodoptera frugiperda | subunit HA1 + subunit HA2 | - |
? | |
| 3.4.21.75 | additional information | LRR peptidyl-7-amido-4-methyl-coumarin is no substrate | Bos taurus | ? | - |
? | |
| 3.4.21.75 | additional information | LRR peptidyl-7-amido-4-methyl-coumarin is no substrate | Spodoptera frugiperda | ? | - |
? | |
| 3.4.21.75 | additional information | plays an important role in posttranslatioal protein processing | Spodoptera frugiperda | ? | - |
? | |
| 3.4.21.75 | peptidyl-7-amido-4-methyl-coumarin + H2O | - |
Bos taurus | 7-amino-4-methyl-coumarin + peptide | - |
? | |
| 3.4.21.75 | peptidyl-7-amido-4-methyl-coumarin + H2O | - |
Spodoptera frugiperda | 7-amino-4-methyl-coumarin + peptide | - |
? | |
| 3.4.21.75 | RTKR 4-methyl-coumarin 7-amide + H2O | - |
Bos taurus | 7-amino-4-methyl-coumarin + RTKR | - |
? | |
| 3.4.21.75 | RTKR 4-methyl-coumarin 7-amide + H2O | - |
Spodoptera frugiperda | 7-amino-4-methyl-coumarin + RTKR | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.21.75 | Bfurin | - |
Bos taurus |
| 3.4.21.75 | Sfurin | - |
Spodoptera frugiperda |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.75 | 40 | - |
rapidly inactivated by increasing temperatures above | Bos taurus |
| 3.4.21.75 | 60 | - |
stable up to | Spodoptera frugiperda |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.75 | 6.5 | 7.5 | - |
Bos taurus |
| 3.4.21.75 | 7.5 | 9 | - |
Spodoptera frugiperda |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.21.75 | 6 | 11 | - |
Bos taurus |
| 3.4.21.75 | 6 | 11 | - |
Spodoptera frugiperda |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
