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Literature summary extracted from

  • Capitani, G.; Eliot, A.C.; Gut, H.; Khomutov, R.M.; Kirsch, J.F.; Grutter, M.G.
    Structure of 1-aminocyclopropane-1-carboxylate synthase in complex with an amino-oxy analogue of the substrate: implications for substrate binding (2003), Biochim. Biophys. Acta, 1647, 55-60.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.14 crystal structure of ACC synthase in complex with the substrate analogue [2-(aminooxy)ethyl](5'deoxyadenosin-5'-yl)(methyl)sulfonium at 2.01 A resolution, crystals are obtained with the sitting drop method, 0.001 ml of protein solution, consisting of 20 mg/ml ACC synthase, 10 mM [2-(aminooxy)ethyl](5'deoxyadenosin-5'-yl)(methyl)sulfonium, 50 mM HEPES, pH 7.9, 0.01 mM pyridoxal 5'-phosphate, 1 mM dithiothreitol, is mixed with 0.001 ml of precipitating solution containing 30% 2-methyl-2-4-pentanediol and 50 mM MES, pH 6.5 Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.14 S-adenosyl-L-methionine Malus domestica
-
 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.14 Malus domestica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.14 recombinant truncated form of ACC synthase Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.14 S-adenosyl-L-methionine
-
 Malus domestica 1-aminocyclopropane-1-carboxylate + methylthioadenosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.4.1.14 ACC synthase
-
 Malus domestica

Cofactor

EC Number Cofactor Comment Organism Structure
4.4.1.14 pyridoxal 5'-phosphate
-
 Malus domestica