Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reichert, A.; Dennes, A.; Vetter, S.; Scheibe, R.
    Chloroplast fructose 1,6-bisphosphatase with changed redox modulation: comparison of the Galdieria enzyme with cysteine mutants from spinach (2003), Biochim. Biophys. Acta, 1645, 212-217.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.11 expression in Escherichia coli Spinacia oleracea
3.1.3.11 expression in Escherichia coli, in this sequence of enzyme, the Cys that corresponds to Cys179 in the spinach enzyme is lacking Galdieria sulphuraria

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.11 C155S mutant is active Spinacia oleracea
3.1.3.11 C174S mutant is active and can be inactivated by oxidation Spinacia oleracea
3.1.3.11 C179S mutant is inactive Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.11 chloroplast
-
 Spinacia oleracea 9507
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.11 Mg2+ required, comparison of activities of wild-type and mutant enzymes in dependence of Mg2+ concentrations Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O Galdieria sulphuraria enzyme is a part of Calvin cycle D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-diphosphate + H2O Spinacia oleracea enzyme is a part of Calvin cycle D-fructose 6-phosphate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Arabidopsis thaliana
-
-
-
3.1.3.11 Brassica napus
-
-
-
3.1.3.11 Galdieria sulphuraria Q95AJ2 red alga
-
3.1.3.11 Glycine max
-
-
-
3.1.3.11 Oryza sativa
-
 rice
-
3.1.3.11 Pisum sativum
-
 pea
-
3.1.3.11 Solanum tuberosum
-
 potato
-
3.1.3.11 Spinacia oleracea
-
-
-
3.1.3.11 Triticum aestivum
-
 wheat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.11
-
 Spinacia oleracea
3.1.3.11
-
 Galdieria sulphuraria

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.11 D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate the chloroplast enzyme of higher plants can occur in an active form that is inactivated upon disulfide bridge formation between Cys155 and Cys174 Spinacia oleracea
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.11 additional information
-
 comparison of activities of oxidizied and reduced enzyme Galdieria sulphuraria
3.1.3.11 additional information
-
 comparison of activities of oxidizied and reduced wild-type and mutant enzymes Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Galdieria sulphuraria D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-bisphosphate + H2O enzyme is a part of Calvin cycle Galdieria sulphuraria D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.11 D-fructose 1,6-diphosphate + H2O enzyme is a part of Calvin cycle Spinacia oleracea D-fructose 6-phosphate + phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.11 8.5
-
 under reducing conditions Spinacia oleracea
3.1.3.11 8.5
-
 under reducing conditions Galdieria sulphuraria

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.11 additional information
-
 the enzyme under oxidizing conditions or untreated is almost inactive with only a small increase starting at pH 9.0 and above Spinacia oleracea
3.1.3.11 additional information
-
 the pH profile of the oxidized form of enzyme reveals an almost constant activity Galdieria sulphuraria